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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(2): 927-937
DOI: https://doi.org/10.20546/ijcmas.2019.802.106


Isolation of Bacteriophages Infecting Ralstonia solanacearum causing Bacterial Wilt Disease in Naga Chilli (Capsicum chinense Jacq.)
Parinda Barua* and Palash Deb Nath
Department of Plant Pathology, Faculty of Agriculture, Assam Agricultural University, Jorhat-785013, Assam, India
*Corresponding author
Abstract:

Ralstonia solanacearum is a soil inhabiting devastating bacterium causing wilt disease in several important agricultural crops. It has an extremely wide host range and ranked as the 2nd destructive and economically important plant pathogenic bacterium of the world. Although several substances like the antibiotics are used in wide scale for its management. However due to numerous limitations such as the emergence of antibiotic resistant bacterial strains, horizontal gene transfer and so on has led to the re- thinking and diversion of the focus on the use of bio control management strategies. The use of bacteriophages for the control of bacterial diseases is a fast expanding management strategy over chemical control. In the present study, R. solanacearum isolates from Naga chilli, chilli pepper, brinjal and banana were isolated and characterized by PCR using specific primers (759/760). Seven lytic bacteriophages were isolated from soil infecting R. solanacearum isolate from Naga chilli (F3C2). After three rounds of purification, the characterization of the phages on the basis of plaque morphology and phage titer was carried out. The host range analysis of the isolated phages revealed that these isolated phages were not strain specific but perhaps somewhat race specific in nature. The results of this research shall serve as a basis towards phage based management strategies against the bacterial wilt disease.


Keywords: Bacteriophages, Ralstonia solanacearum, bacterial wilt, Naga Chilli (Capsicum chinense Jacq.)
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How to cite this article:

Parinda Barua and Palash Deb Nath. 2019. Isolation of Bacteriophages Infecting Ralstonia solanacearum causing Bacterial Wilt Disease in Naga Chilli (Capsicum chinense Jacq.).Int.J.Curr.Microbiol.App.Sci. 8(2): 927-937. doi: https://doi.org/10.20546/ijcmas.2019.802.106