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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(1): 3184-3193
DOI: https://doi.org/10.20546/ijcmas.2019.801.340


Investigation of antibiofilm potential of Argemone mexicana and Calotropis gigantea against clinical isolates
R. Ramya and S. M. Gopinath*
Research Center, Department of Biotechnology, Acharya Institute of Technology, Bengaluru, Karnataka, India- 560 107
*Corresponding author
Abstract:

In the current investigation, the anti-biofilm potential of Argemone mexicana and Calotropis gigantea against 11 clinical isolates viz Escherichia coli, Pseudomonas aeruginosa, Enterococcus, Enterobacter aerogenes, Klebsiella pneumoniae, Staphylococcus aureus, Proteus vulgaris, Proteus mirabilis, Citrobacter, Serratia marcescens and Staphylococcus epidermidis were tested. The phytochemicals extracted in different solvents were analysed for their antibiofilm ability. For detection of biofilm formation, 11 clinical isolates were screened by Tube method (TM), Congo red agar (CRA), Static Glass coupon reactor method and invitro catheter method. Of the 11isolates, 7(57.8%) displayed a biofilm-positive phenotype under the optimized conditions in the TCP method and strains were further classified as strong 3 (27.27 %) and moderate 4 (36.36 %) while in 4 (36.36 %) isolates weak or no biofilm was detected. Though TM correlated well with the CRA test for 3 (27.3 %) strongly biofilm producing strains, weak producers were difficult to discriminate from biofilm negative isolates. Screening on Static Glass coupon reactor does not correlate well with either of the two methods for detecting biofilm formation in clinical isolates. Methanolic extract of Argemone mexicana consistently showed inhibitory effect against the E. coli, P. vulgaris and P. aeruginosa, while acetone extract of Argemone mexicana showed inhibitory effect against S. aureus and Enterococcus. Calotropis gigantea however was less efficient when compared to Argemone mexicana.


Keywords: A. mexicana, C. gigantea, clinical isolates, biofilm, MDR
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How to cite this article:

Ramya, R. and Gopinath, S. M. 2019. Investigation of antibiofilm potential of Argemone mexicana and Calotropis gigantea against clinical isolates.Int.J.Curr.Microbiol.App.Sci. 8(1): 3184-3193. doi: https://doi.org/10.20546/ijcmas.2019.801.340