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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(2): 297-308
DOI: http://dx.doi.org/10.20546/ijcmas.2016.502.034


Purification and Biochemical Characteristic of Protease from the Red Seaweed Petrocladia capillacea
Hamed M El-Shora*, G.S. Abou-El-Wafa and A.I. Kadhim
Botany Department, Faculty of Science, Mansoura University, Egypt
*Corresponding author
Abstract:

The aims of the present work was to purify protease enzyme from the redsea weed Petrocladia capillacea. Protease was purified by 70 % ammonium sulfate and Sephadex G-200. The specific activity was 280 units mg-1 protein and with 122-fold.The optimal casein concentration was 40% (w/v). The Km and Vmax values were 52.6 % (w/v) and 33.3 units mg-1protein.The final incubation time was 40 min. The optimal pH and optimal temperature were 7 and 40ºC. Ca+2 and Mg+2 were the best activator divalent cations. The other cations Hg2+, Cu2+, Al3+, Co2+ and Zn2+were inhibitors. Protease was activated by the amino acids cysteine and methionine. However, protease was activated by reduced glutathione, thiourea, thioglycolate, and acetyl cysteine.


Keywords: Petrocladia capillacea, Biochemical Characteristic of Protease
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How to cite this article:

Hamed M El-Shora, G.S. Abou-El-Wafa and A.I. Kadhim. 2016. Purification and Biochemical Characteristic of Protease from the Red Seaweed Petrocladia capillacea.Int.J.Curr.Microbiol.App.Sci. 5(2): 297-308. doi: http://dx.doi.org/10.20546/ijcmas.2016.502.034