International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 5, Issue:2, February, 2016

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(2): 297-308

Purification and Biochemical Characteristic of Protease from the Red Seaweed Petrocladia capillacea
Hamed M El-Shora*, G.S. Abou-El-Wafa and A.I. Kadhim
Botany Department, Faculty of Science, Mansoura University, Egypt
*Corresponding author

The aims of the present work was to purify protease enzyme from the redsea weed Petrocladia capillacea. Protease was purified by 70 % ammonium sulfate and Sephadex G-200. The specific activity was 280 units mg-1 protein and with 122-fold.The optimal casein concentration was 40% (w/v). The Km and Vmax values were 52.6 % (w/v) and 33.3 units mg-1protein.The final incubation time was 40 min. The optimal pH and optimal temperature were 7 and 40ºC. Ca+2 and Mg+2 were the best activator divalent cations. The other cations Hg2+, Cu2+, Al3+, Co2+ and Zn2+were inhibitors. Protease was activated by the amino acids cysteine and methionine. However, protease was activated by reduced glutathione, thiourea, thioglycolate, and acetyl cysteine.

Keywords: Petrocladia capillacea, Biochemical Characteristic of Protease

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How to cite this article:

Hamed M El-Shora, G.S. Abou-El-Wafa and A.I. Kadhim. 2016. Purification and Biochemical Characteristic of Protease from the Red Seaweed Petrocladia capillacea.Int.J.Curr.Microbiol.App.Sci. 5(2): 297-308. doi:
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