National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The aims of the present work was to purify protease enzyme from the redsea weed Petrocladia capillacea. Protease was purified by 70 % ammonium sulfate and Sephadex G-200. The specific activity was 280 units mg-1 protein and with 122-fold.The optimal casein concentration was 40% (w/v). The Km and Vmax values were 52.6 % (w/v) and 33.3 units mg-1protein.The final incubation time was 40 min. The optimal pH and optimal temperature were 7 and 40ºC. Ca+2 and Mg+2 were the best activator divalent cations. The other cations Hg2+, Cu2+, Al3+, Co2+ and Zn2+were inhibitors. Protease was activated by the amino acids cysteine and methionine. However, protease was activated by reduced glutathione, thiourea, thioglycolate, and acetyl cysteine.
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