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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(1): 2740-2748
DOI: https://doi.org/10.20546/ijcmas.2019.801.290


Effect of Tris Egg Yolk and Tris Soya Lecithin Extenders in Frozen Beetal Buck Semen
D. Baruah1*, S. Sinha1, B.C. Deka1, R.K. Biswas1, R.S. Borah2 and A. Saleque3
1Department of Animal Reproduction, Gynaecology & Obstetrics, College of Veterinary Science, AAU, Khanapara, Guwahati-22, India
2Department of L.P.M. (Biostat) College of Veterinary Science, AAU, Khanapara, Guwahati-22, India
3Goat Research Station, A. A. U., Burnihat, India
*Corresponding author
Abstract:

The aim of this study was to evaluate the effect of Tris egg yolk and Tris soya lecithin in extenders for goat sperm cryopreservation. A total of 15 pooled ejaculates from five adult Beetal bucks maintained at Goat Research Station, AAU, Burnihat, were used in the study. Immediately after collection each ejaculate was evaluated for volume, mass activity and initial sperm motility and those found suitable were pooled. The pooled ejaculates were split into two equal parts and extended with two extenders i.e., Tris extender containing 20 per cent egg yolk (TEY) and Tris extender supplemented with 1.5 per cent soya lecithin (TSL). The extended semen was filled in 0.25 ml French straws and frozen using liquid nitrogen vapour. The mean post-thaw sperm motility, live sperm and intact acrosome in Tris extender containing 20 per cent egg yolk was significantly higher than that in Tris extender containing 1.5 per cent soya lecithin. The mean post-thaw HOST-reacted spermatozoa was non significant between Tris extenders containing 20 per cent egg yolk and 1.5 per cent soya lecithin. In conclusion, although soya lecithin represent an alternative chemically defined extender with decrease risk of biological contamination, egg yolk based extenders are more efficient for cryopreservation of goat semen.


Keywords: Tris soya lecithin, Soya lecithin, Cryopreservation, Buck, Host-reacted, French straw
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How to cite this article:

Baruah, D., S. Sinha, B.C. Deka, R.K. Biswas, R.S. Borah and Saleque, A. 2019. Effect of Tris Egg Yolk and Tris Soya Lecithin Extenders in Frozen Beetal Buck Semen.Int.J.Curr.Microbiol.App.Sci. 8(1): 2740-2748. doi: https://doi.org/10.20546/ijcmas.2019.801.290