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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(1): 2610-2619
DOI: https://doi.org/10.20546/ijcmas.2019.801.274


Direct in vitro Regeneration of Gerbera (Gerbera jamesonii Bolus)
C.L. Vijayalakshmi1*, P. Babu2, A.N. Bagali1, C.D. Soregaon3 and Ashwini H. Wadageri1
1Department of Horticulture, 3Department of Genetics and Plant Breeding, College of Agriculture, Vijayapur
2Department of Horticulture, College of Agriculture, Hanumanamatti, University of Agricultural Sciences, Dharwad - 580 005, Karnataka, India
*Corresponding author
Abstract:

Gerbera (Gerbera jamesonii Bolus) seeds were used as primary source of explants for sterilization. Seeds were sterilized with 0.1 % mercuric chloride for 2 minutes to obtain minimum contamination (10 %). MS (Murashige and Skoog’s media) supplemented with 4 mg per litre BAP + 0.3 mg per litre IAA served as the best medium for shoot proliferation from in vitro shoots as it produced maximum number of shoots (2.67) with maximum number of leaves (4.73) and maximum shoot length (1.92 cm) at three weeks of inoculation. For root regeneration MS media supplemented with 2 mg per litre IBA was recorded as the best rooting media as regenerated roots early (10.87) with maximum number of roots (4.73) and maximum root length (3.47 cm).


Keywords: Gerbera jamesonii, sterilization, BAP (6-benzyl amino purine), IAA (indole-3-acetic acid)
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How to cite this article:

Vijayalakshmi, C.L., P. Babu, A.N. Bagali, C.D. Soregaon and Ashwini H. Wadageri. 2019. Direct in vitro Regeneration of Gerbera (Gerbera jamesonii Bolus).Int.J.Curr.Microbiol.App.Sci. 8(1): 2610-2619. doi: https://doi.org/10.20546/ijcmas.2019.801.274