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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2016: 92.3
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3
For more details click here
journals IJCMAS
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(12): 36-55
DOI: http://dx.doi.org/10.20546/ijcmas.2016.512.005


Influence of α-Pinene and Pine Needles of  Pinus sylvestris (L) on the Cocoons and Silk Fibres Spinned by Fifth Instar Larvae of Silkworm, Bombyx mori (L) (Race: PM x CSR2)
Vitthalrao B. Khyade1*, Vishakha S. Chape2 and Priyanka B. Ghadge2
1Sericulture Unit, Malegaon Sheti Farm, Agricultural Development Trust Baramati, Shardanagar, (Malegaon Khurd) Post Box No.- 35 Tal. Baramati, Dist. Pune 413 115 Maharashtra, India
2Department of Botany, Shardabai Pawar Mahila Mahavidyalaya, Shardanagar, Malegaon (Baramati) Dist. Pune – 413115, India
*Corresponding author
Abstract:

The pinene has  been used as anti-cancer agent in Traditional Chinese medicine, also for its anti-inflammatory, antiseptic, expectorant and bronchodilator properties. Insect juvenoid activity of Terpenes and terpene contents of Pine needles of Pinus sylvestris (L) are  well established facts. Topical application of ten microliters of one milligram per liter strength acetone solution of α-Pinene and Pine needles to the individual fifth instar larvae of multivoltine cross breed race of silkworm, Bombyx mori ( L ) ( PM x CSR2)  at 48, 54, 60 and 66 hours after the fourth moult was found variously reflected into the prolongation of larval duration (17.011– 45.394 % for α-Pinene  treated groups and 19.078 – 48.684 % for Pine needle treated groups); improvement in the tissue somatic index (TSI) of silk glands (3.054 – 3.344 for α-Pinene treated groups and 3.071 – 3.094 % for Pine needle treated groups); cocoon shell ratio (2.791 – 3.179 for α-Pinene treated groups and 2.694 – 3.631  for Pine needle treated groups) and denier scale of silk filament (0.333 – 2.359 for α-Pinene treated group and 0.466 – 2.405 for Pine needle treated group). Acetone solution of α-Pinene and acetone solution of pine needles, thus chiefly lengthening the larval duration (age) in silkworm, Bombyx mori  (L).  The lengthened larval life is sufficient to prove the juvenoid activity of acetone solution of Pine Needles. Treated group of larvae may be utilizing the lengthened life span for the fortification of cocoon shell through the silk filament. The synergistic activity of α-Pinene and Pine needles in the present attempt is hypothesized to be due to changes in the membrane fluidity, interference with membrane bound signaling proteins and cell cycle arrest.   Efficient utilization of α-Pinene and pine needles,  through acetone solvent for topical application to the fifth instared larvae of silkworm, Bombyx mori (L) may open a new innovative biotechnological avenue in the sericulture industries.


Keywords: α-Pinene;Pine needles ;TSI ;Shell Ratio;Denier Scale of Silk Filament.
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How to cite this article:

Vitthalrao B. Khyade, Vishakha S. Chape and Priyanka B. Ghadge. 2016. Influence of α-Pinene and Pine Needles of  Pinus sylvestris (L) on the Cocoons and Silk Fibres Spinned by Fifth Instar Larvae of Silkworm, Bombyx mori (L) (Race: PM x CSR2).Int.J.Curr.Microbiol.App.Sci. 5(12): 36-55. doi: http://dx.doi.org/10.20546/ijcmas.2016.512.005