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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(1): 2289-2304
DOI: https://doi.org/10.20546/ijcmas.2019.801.240


Genome Sequence Analysis and Identification of Genes Associated to Pesticide Degradation from Enterobacter cloacae Strain MR2
M.V. Parakhia*, R.S. Tomar, H. Dalal, V.V. Kothari, V.M. Rathod and B.A. Golakiya
Department of Biotechnology, Junagadh Agricultural University, Junagdah-362001, Gujarat, India
*Corresponding author
Abstract:

Today's burning problem in the world is pesticide residues in foods. To overcome this problem, nineteen chlorpyrifos-degrading bacteria were isolated from soil of adjoining area of pesticide manufacturing industries located in Gujarat, India. The strain CPD-12 (MR2) degraded highest chlorpyrifos among the other strains isolated from different sites, i.e. Up to 500 ppm in 30 hrs. And hence was selected further for whole genome sequencing. This strain showed maximum similarity to members of the order Enterobacteriales and was closest to Enterobacter cloacae of this group. The genome sequence of strain Enterobacter cloacae MR2 consisted of a circular 4,758,062bp chromosome with a 55.1% G +C value, 5571 protein coding genes, 16rRNA and 72 tRNAs. The genome annotation and functional characterization of the strain MR2 provided insights into various genetic processes involved in the degradation of several pesticides and detoxification of toxic compounds. The genome of MR2 was also compared with Enterobacter cloacae subsp cloacae ATCC 13047 and Enterobacter sp. 638 which showed the presence of genes for the pesticide degradation as in ATCC 13047 and also had genes to promote plant growth as in Enterobacter sp. 638.


Keywords: Whole genome sequencing, chlorpyrifos degradation, Enterobacter cloacae MR2, Draft genome
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How to cite this article:

Parakhia, M.V., R.S. Tomar, H. Dalal, V.V. Kothari, V.M. Rathod and Golakiya, B.A. 2019. Genome Sequence Analysis and Identification of Genes Associated to Pesticide Degradation from Enterobacter cloacae Strain MR2.Int.J.Curr.Microbiol.App.Sci. 8(1): 2289-2304. doi: https://doi.org/10.20546/ijcmas.2019.801.240