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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(1): 2061-2076
DOI: https://doi.org/10.20546/ijcmas.2019.801.216


Utilisation of Agrowaste Xylan for the Production of Industrially Important Enzyme Xylanase from Aquatic Streptomyces sp. and Potential Role of Xylanase in Deinking of Newsprint
Emilda Rosmine*, Neethu Changan Edassery Sainjan, Reshma Silvester and Saramma Aikkarakunnath Varghese
Department of Marine Biology, Microbiology and Biochemistry, School of Marine Sciences, CUSAT, Kerala, India
*Corresponding author
Abstract:

Xylanase is an industrially significant enzyme and its production from pure xylan is expensive. The objective of the current study is to utilise sustainable cost effective substrates -coconut oil cake, corn cob, sugarcane bagasse and water hyacinth, for xylanase production. Streptomyces sp. ER1 isolated from the sediments of Cochin estuary was used for xylanase production. The cultural and nutritional conditions for higher xylanase production using the four substrates were optimised using one factor at a time method. Data were analysed by one way ANOVA. The maximum xylanase yield was observed for sugarcane bagasse (10533.33 U/mL), corn cob (7880.9 U/mL) followed by, coconut oil cake (7680 U/mL) and water hyacinth (6930 U/mL) in submerged fermentation. Optimisation studies revealed that optimum fermentation and nutritional factors varied with the substrate. The crude xylanase was vastly effective in deinking of the newspaper at elevated temperature. This study proved that utilising agrowastes provides cost effective and eco-friendly method for xylanase production on large scale. Thus it is an alternative approach to reducing environmental pollution caused due to dumping agro waste. No studies on xylanaolytic activity of actinomycetes from Cochin estuary has been done so far.


Keywords: Xylanase, Streptomyces, Fermentation, Optimisation, Agrowastes, Deinking
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How to cite this article:

Emilda Rosmine, Neethu Changan Edassery Sainjan, Reshma Silvester and Saramma Aikkarakunnath Varghese. 2019. Utilisation of Agrowaste Xylan for the Production of Industrially Important Enzyme Xylanase from Aquatic Streptomyces sp. and Potential Role of Xylanase in Deinking of Newsprint.Int.J.Curr.Microbiol.App.Sci. 8(1): 2061-2076. doi: https://doi.org/10.20546/ijcmas.2019.801.216