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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(11): 829-835
DOI: http://dx.doi.org/10.20546/ijcmas.2016.511.094


Preparation and Evaluation of Bacteriophage Lysate Specific for Salmonella  typhimurium
Adeela Iqbal*, M. Shafi Hasni, Sajjad-ur-Rahman, Rizwan Aslam and Kasib Khan
University of Agriculture, Faisalabad, Pakistan
*Corresponding author
Abstract:

Salmonella is Gram negative, rod-shaped bacteria S. typhimurium not only infects the humans but also infects the animal and poultry. Phage therapy is a method of treatment in which viruses are used to lyses the bacteria. The aim of this study was growth of bacteriophages against S. typhimurium.26 samples were collected from University of Agriculture Faisalabad out of which 5 samples were from poultry droppings, 5samples were from cow fecal, 6 stool samples were from cat, 5 stool samples were from sheep and horse. When these samples were cultured in Tetrathionate broth 21 samples showed turbidity. These 21 samples were cultured on Salmonella Shigella agar. 18samples showed colorless colonies on SS agar. These colonies were further streaked on Hardy CHROME SS agar after 24h incubation at 37oC large black and clear center colonies appeared on this media. Sewage water contains abundant S.Typhimurium serves as vulnerable and extremely flexible host for the development of phages, so 5 sewage water samples were collected. The plaque caused by the phages varies in size and is characterized by spherical zone. The number of PFU from phage lysate was ranging from 105 ×106pfu/ml.


Keywords: typhimurium, Salmonellosis, bacteriophages, Hardy Chrome SS agar.
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How to cite this article:

Adeela Iqbal, M. Shafi Hasni, Sajjad-ur-Rahman, Rizwan Aslam and Kasib Khan. 2016. Preparation and Evaluation of Bacteriophage Lysate Specific for Salmonella typhimurium.Int.J.Curr.Microbiol.App.Sci. 5(11): 829-835. doi: http://dx.doi.org/10.20546/ijcmas.2016.511.094