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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(12): 3705-3713
DOI: https://doi.org/10.20546/ijcmas.2018.712.422


Serodetection of Hydatidosis in Buffaloes using Recombinant Ag-B Subunit 8 of Echinococcus granulosus
Zamir Ali Ganaie1*, S. Samanta2, Mary Nisha Tigga2, Ajayta Rialch2,Lalrin Kima2, M. Norjit Singh2 and O.K. Raina2
1Division of Veterinary Parasitology, F.V.Sc & A.H, SKUAST-Kashmir 190006, India 2Division of Veterinary Parasitology, ICAR-IVRI, Izatnagar, Bareilly, U.P 243122, India
*Corresponding author
Abstract:

Hydatidosis caused by metacestode stage of Echinococcus granulosus is one of the most important cyclozoonotic disease affecting both humans and livestock resulting in considerable economic losses to livestock industry which amounts to US$ 3 billion annually (WHO factsheet on Echinococcosis, 2018). No reliable immunodiagnostic method is available for its sero-detection in animals. Native antigen-B represents one of the most immuno-dominant antigens in hydatidosis, therefore assessment of recombinant antigen-B subunit 8 was taken in the current study. The immuno-reactivity of recombinant 29-kDa EgAgB-8 protein was confirmed in the Western blot with rabbit anti-histidine antibodies and buffalo sera positive for hydatid infection. The sero-diagnostic potential of this recombinant protein was evaluated by indirect ELISA and DOT-ELISA platforms by screening two hundred and fifty buffalo sera where 44.8% (n=112) samples were revealed positive by indirect-ELISA by detecting antibodies against this antigen in sera. Immuno-reactivity with hydatid positive buffaloes (n=12) was also confirmed in DOT-ELISA. Cross-reactivity of the recombinant antigen with field sera positive for Gigantocotyle explanatum, Paramphistomum epiclitum and Gastrothylax crumenifer was also studied in DOT-ELISA that showed some of the buffaloes infected with parasites other than hydatid metacestode were cross-reacting with the recombinant antigen.


Keywords: Cystic Echinococcosis, DOT-ELISA, Immunoreactivity, Serodiagnosis
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How to cite this article:

Zamir Ali Ganaie, S. Samanta, Mary Nisha Tigga, Ajayta Rialch, Lalrin Kima, M. Norjit Singh, Raina, O.K. 2018. Serodetection of Hydatidosis in Buffaloes using Recombinant Ag-B Subunit 8 of Echinococcus granulosus.Int.J.Curr.Microbiol.App.Sci. 7(12): 3705-3713. doi: https://doi.org/10.20546/ijcmas.2018.712.422