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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(11): 681-690
DOI: http://dx.doi.org/10.20546/ijcmas.2016.511.079


Characterization of Lipase from Wild (LPF-5) and Mutant (HN1) Strain of Aspergillus nigera
Arun Kumar Sharma1, Vinay Sharma1* and Jyoti Saxena2
1Department of Bioscience and Biotechnology, Banasthali University, Rajasthan, India
2Department of Biochemical Engineering, Bipin Tripathi Kumaon Institute of Technology, Dwarahat, Uttrakhand, India
*Corresponding author
Abstract:

Lipase is one of the most imperative industrial enzymes and has a variety of applications in various industries. In the present study, lipases obtained by submerged cultivation of wild (LPF-5) and mutant (HN1) strain of A. niger were used and compared for characterization study. Activity and stability of partially purified lipase was determined under different pH, temperature, organic solvents and metal ions. The lipase showed highest activity and stability at pH 7.0 and temperature 35 °C for LPF-5 and 30 °C for HN1 strain. The lipases retained high activity over ranges of temperature (25-50 ºC) and pH (4.0-7.5). Lipase was enhanced by methanol and acetone, while slightly inhibited by butanol (10% v/v). Ca2+ appeared to be the excellent inducer of lipase activity. Lipase also showed stability in presence of the other metal ions (Na+, Ba2+, Mg2+, Cu2+, Fe2+ and Mn2+). The lipase of wild and mutant strain retained 10.58% and 14.60% of its activity when pre-incubated with Hg2+, indicating the inhibitory effect of Hg2+ on catalytic activity of lipase.


Keywords: A. niger, mutant strain (HN1),wild strain (LPF-5), temperature, pH, metal ions, methanol, characterization
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How to cite this article:

Arun Kumar Sharma, Vinay Sharma and Jyoti Saxena. 2016. Characterization of Lipase from Wild (LPF-5) and Mutant (HN1) Strain of Aspergillus nigerInt.J.Curr.Microbiol.App.Sci. 5(11): 681-690. doi: http://dx.doi.org/10.20546/ijcmas.2016.511.079