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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(11): 648-655
DOI: http://dx.doi.org/10.20546/ijcmas.2016.511.076


Fluconazole Resistance Pattern of Candida isolates in Clinical Samples by E-Test
V. Mangayarkarasi1*, Kanishka Hrishi Das1, Shabana Praveen2, V.Chitraleka2 and Christopher Amalraj3
1Department of Microbiology, SRM medical college hospital and research centre (SRM MCH&RC), Kancheepuram, Tamil Nadu, India
2Department of Cardiology, SRM medical college hospital and research centre (SRM MCH&RC), Kancheepuram, Tamil Nadu, India
3Department of Community medicine, SRM medical college hospital and research centre
(SRM MCH&RC), Kancheepuram, Tamil Nadu, India
*Corresponding author
Abstract:

Candida species are often present as commensal organisms in the healthy individuals, the emergence of Candida species which are resistance to fluconazole has become a great concern globally. Aim of this study was to demonstrate fluconazole resistant pattern of Candida species isolated in various clinical samples. This discriptive Study was carried out in SRM Medical College Hospital & Research centre, Kattankulathur, Kanchipuram, Tamil Nadu, India, between January 2014-2015. Candida species were identified by culture on Sabouraud dextrose agar and confirmed by Gram staining, Germ tube test, Sugar assimilation and fermentation test. Fluconazole resistance was detected by Epsilometric test (E-Test) and the minimum inhibitory concentration (MIC) of the Candida species was measured. Chi square test were used for statistical analysis. A total of 98 Candida species were isolated from 168 clinical specimens, 42.8% of Candida albicans and 57.2% of Candida non albicans were detected, of the 98 isolates 23.4% of Candida species were resistant to fluconazole, highest resistance was seen in Candida krusei (90%). More number of candida non albicans were isolated and showed resistance to fluconazole than candida albicans, hence Candida non albicans should be considered in patient as pathogen and clinically correlate with the infection and presentation of the patient.


Keywords: Candida albicans, Candida non albicans, Epsilometric test, Fluconazole Resistance,Minimum inhibitory concentration.
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How to cite this article:

Mangayarkarasi, V., Kanishka Hrishi Das, Shabana Praveen, V. Chitraleka and Christopher Amalraj. 2016. Fluconazole Resistance Pattern of Candida isolates in Clinical Samples by E-Test.Int.J.Curr.Microbiol.App.Sci. 5(11): 648-655. doi: http://dx.doi.org/10.20546/ijcmas.2016.511.076