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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(12): 2616-2620
DOI: https://doi.org/10.20546/ijcmas.2018.712.296


Isolation and Identification of Nematicidal Principle in Lippia nodiflora against Root Knot Nematode Meloidogyne incognita
T. Senthilkumar* and S. Ramakrishnan
Department of Nematology, TNAU, Coimbatore - 641003
*Corresponding author
Abstract:

The protein and non protein parts present in the leaves of Lippia nodiflora were separated and test their nematicidal activity against egg hatching and juvenile mortality of Meloidogyne incognita. The non protein part was found to inhibit the root knot nematode egg hatching under in vitro conditions. Leaf extraction was done with different solvent such as petroleum ether, chloroform methanol using soxhlet apparatus and the residue was discarded. Thin layer chromatography method was used to isolate and identify the nematicidal principle present in Lippia nodiflora. The identified compounds in non protein parts such as phenols, alkaloids and terpenoids were tested for their nematicidal activity on egg hatching and mortality of M. incognita juveniles. The nematicidal assay of these compounds revealed that the alkaloids inhibited egg hatching absolutely and caused juvenile mortality followed by phenols. The terpenoids did not show any marked inhibition on hatching of eggs and causing mortality of juveniles of M. incognita.


Keywords: Lippia nodiflora, Nematicidal compounds, Phenols, Alkaioids, root knot nematode, Meloidogyne incognita
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How to cite this article:

Senthilkumar, T. and Ramakrishnan, S. 2018. Isolation and Identification of Nematicidal Principle in Lippia nodiflora against Root Knot Nematode Meloidogyne incognita.Int.J.Curr.Microbiol.App.Sci. 7(12): 2616-2620. doi: https://doi.org/10.20546/ijcmas.2018.712.296