Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(11): 604-617
DOI: http://dx.doi.org/10.20546/ijcmas.2016.511.071


Radial Growth of three Paecilomyces Species Isolated  from Two Ghanaian Maize Varieties Abeleehi and Obaatanpa on Five Different Media and the Effects of their Culture Filtrate on Seed Germination and Radicle Elongation of Abeleehi and Obaatanpa
Andrew A. Minamor1* and G.T. Odamtten2
1Department of Science Laboratory Technology, Accra Technical University,O. Box GP 561, Accra- Ghana
2Department of Plant and Environmental Biology, School of Biological Sciences,University of Ghana, P. O. Box LG 55 Legon
*Corresponding author
Abstract:

Three xerophilic fungal species, Paecilomyces carneus, P. puntoni, and P. varioti were for the first time isolated from two newly-developed Ghanaian maize varieties; Abeleehi and Obaatanpa. The fungal species were isolated under varying ambient humidity of 55-65% in both grain varieties. The culture filtrate of the Paecilomyces species raised in Maize Meal broth prepared from either Abeleehi or Obaatanpa and Potato-Dextrose broth were tested on the germination capacity of grains of Abeleehi and Obaatanpa using the blotter test method. The physiological condition for optimal growth of the Paecilomyces species were tested on Czapek-Dox Agar, Maize Meal Agar (Abeleehi), Maize Meal Agar (Obaatanpa), Malt Extract Agar and Potato Dextrose Agar at  (18, 30, 35, and 40) oC. Radial growth of the Paecilomyces species was influenced  by media and temperature of incubation. The optimum growth of P. carneus on all agar media used was obtained at a 30oC, a temperature of 40oC was clearly unsuitable for growth of P. carneus as the fungus remained static at this temperature after 2-4 days growth. The best radial growth of P. puntoni was obtained between 30-35oC. Growth at 18oC initially lagged behind those kept at 30oC but approximated their growth rate after 6 days incubation period. There was no statistical difference (P ≤ 0.05) between growth of P. puntoni in all the media tested. Temperature of 35oC and 40oC depressed radial growth of P. varioti in all media tested. The best temperature for growth of P. varioti was 30oC. Temperature 18oC was almost as suitable as 30oC for P. varioti. Radial growth of P. varioti was slowest on Potato Dextrose agar as compared to the other media tested. Percentage germination of the maize grains was depressed by 10 -75% by the undiluted, 2, 4, and 8 days old culture filtrate of the three Paecilomyces species. However, there were varietal differences in the response of the germinating maize grains to the active ingredient in the culture metabolites of the three Paecilomyces species. The inhibitory effect was gradually removed with increasing dilution. The same culture filtrate severely depressed length of emerging radicles of the two maize varieties by 40 - 90% at the highest concentration applied. The reduction in radicle length was severer on Abeleehi variety as compared to Obaatanpa.


Keywords: Abeleehi, Obaatanpa,Maize Meal Agar, Paeclomyces, Radicle length.
Download this article as Download

How to cite this article:

Andrew A. Minamor and G.T. Odamtten. 2016. Radial Growth of three Paecilomyces Species Isolated  from Two Ghanaian Maize Varieties Abeleehi and Obaatanpa on Five Different Media and the Effects of their Culture Filtrate on Seed Germination and Radicle Elongation of Abeleehi and ObaatanpaInt.J.Curr.Microbiol.App.Sci. 5(11): 604-617. doi: http://dx.doi.org/10.20546/ijcmas.2016.511.071