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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(12): 2177-2183
DOI: https://doi.org/10.20546/ijcmas.2018.712.247


Evaluation of Lactic Acid Bacteria, Clostridium and Paenibacillus Isolates for 2, 3-Butanediol Production and Process Development using Agro-residues
M. Ashwini* and Geeta Shirnalli
AICRP ON EAAI (Bioconversion Technology, Main Agriculture Research Station, University of Agricultural Sciences, Dharwad-580 00, Karnataka, India
*Corresponding author
Abstract:

2,3-Butanediol (2,3-BD) is one of the promising bulk chemicals due to its extensive industrial application. We need to exploit the innate potential of its microbial producers which also possess delignification and saccharification abilities to facilitate the use of renewable and cheaper agro-residues as raw materials for fermentation. Strains of Lactic acid bacteria, Paenibacillus and Clostridium were tested for production of 2,3-BD by high through put determination of a pathway intermediate acetoin by Vogues Proskauer test. The VP positive isolates were grown as shaken flask cultures and the concentration of the accumulated 2,3 BD was determined by Gas chromatography. The highest amount of 18.577 g 2,3 BD /L was produced by the LAB isolate LDL-18, followed by other LAB isolates LA-3 and LGr-5 which produced 1.3908 and 1.9893 g/L of 2,3-BD respectively. Both the Panebacillus strains showed very less production of 2, 3 BD. Further the possibility of producing 2,3-BDL was tested by using agro-residues viz., pineapple pulp waste and delignified paddy straw at different concentrations in batch fermentation. This study is a prelude to an alternate, sustainable energy management strategy to fulfil the increasing demand of this useful chemical and to overcome its expensive chemical synthesis.


Keywords: 2,3-Butanediol, Lactic acid bacteria, Clostridium, Paenibacillus, Vogues Proskauer test, Agro-residues
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How to cite this article:

Ashwini, M. and Geeta Shirnalli. 2018. Evaluation of Lactic Acid Bacteria, Clostridium and Paenibacillus Isolates for 2, 3-Butanediol Production and Process Development using Agro-residues.Int.J.Curr.Microbiol.App.Sci. 7(12): 2177-2183. doi: https://doi.org/10.20546/ijcmas.2018.712.247