National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
In the modern era, there is a lot of concern towards mycology due to an expanding array of fungal infections. Though Lacto Phenol Cotton Blue is widely used for staining, there is a necessity to develop an alternate equipotent stain which can replace it due to its high level of tumorigenic and hazardous nature. The novel stain should be eco friendly, safe with good visual clarity and staining characteristics. With this intention we have analysed a novel staining reagent Iodine-Glycerol. Various clinical samples and fungal stock cultures were analysed simultaneously using Lacto-phenol Cotton Blue and Iodine Glycerol using techniques like teasing technique, slide culture technique and adhesive tape technique. Parameters like degree of transparency, visual clarity, resolution, contrast, staining characteristics like uniformity, formation of artifacts were analysed for better demonstration of fungal morphology. Iodine-Glycerol is a better alternative to Lacto-phenol Cotton Blue for the demonstration of fungal morphology in the clinical microbiology laboratory. It is eco-friendly, non carcinogenic and much potent staining reagent. It is necessary to carry further research as there are no specific guidelines regarding the preparation of the Iodine-Glycerol staining reagent.
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