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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(11): 536-541
DOI: http://dx.doi.org/10.20546/ijcmas.2016.511.063


Efficacy of Iodine-Glycerol versus Lactophenol Cotton Blue for Identification of Fungal Elements in the Clinical Laboratory
Siva Prasad Reddy Basava*, Srijan Ambati, Kandati Jithendra, N. Premanadham, P.Sreenivasulu Reddy and Charan Kumar Mannepuli
Department of Microbiology, Narayana Medical College, Nellore, Andhra Pradesh, India
*Corresponding author
Abstract:

In the modern era, there is a lot of concern towards mycology due to an expanding array of fungal infections. Though Lacto Phenol Cotton Blue is widely used for staining, there is a necessity to develop an alternate equipotent stain which can replace it due to its high level of tumorigenic and hazardous nature. The novel stain should be eco friendly, safe with good visual clarity and staining characteristics. With this intention we have analysed a novel staining reagent Iodine-Glycerol. Various clinical samples and fungal stock cultures were analysed simultaneously using Lacto-phenol Cotton Blue and Iodine Glycerol using techniques like teasing technique, slide culture technique and adhesive tape technique. Parameters like degree of transparency, visual clarity, resolution, contrast, staining characteristics like uniformity, formation of artifacts were analysed for better demonstration of fungal morphology. Iodine-Glycerol is a better alternative to Lacto-phenol Cotton Blue for the demonstration of fungal morphology in the clinical microbiology laboratory. It is eco-friendly, non carcinogenic and much potent staining reagent. It is necessary to carry further research as there are no specific guidelines regarding the preparation of the Iodine-Glycerol staining reagent.


Keywords: LPCB, Iodine-Glycerol, Lugol’s Iodine, Fungal Morphology, Transparency, Staining Characters.
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How to cite this article:

Siva Prasad Reddy Basava, Srijan Ambati, Kandati Jithendra, N. Premanadham, P. Sreenivasulu Reddy and Charan Kumar Mannepuli. 2016. Efficacy of Iodine-Glycerol versus Lactophenol Cotton Blue for Identification of Fungal Elements in the Clinical Laboratory.Int.J.Curr.Microbiol.App.Sci. 5(11): 536-541. doi: http://dx.doi.org/10.20546/ijcmas.2016.511.063