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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(12): 1704-1714
DOI: https://doi.org/10.20546/ijcmas.2018.712.198


Assessing Genetic Diversity using RAPD Molecular markers in Coffea canephora Pierre ex. Froehner (Robusta coffee): A Step towards Crop Improvement
Mallikarjun G. Awati3*, B.S. Tambat2, G.F.D’souza1, D. Venkataramanan1, M. Udaya Kumar3, C.G. Anand1 and Y. Raghuramulu1
1Division of Plant Physiology, Central Coffee Research Institute, Coffee Research Station- 577 117, Chikmagalur District, Karnataka, India
2Department of Crop Physiology, Agricultural College Hassan campus
3GKVK campus, University of Agricultural Sciences, Bengaluru- 560 065, Karnataka, India
3 Department of Biotechnology and Crop Improvement, College of Horticulture, Udyanagiri, UHS, Bagalkot-587104, Karnataka, India
*Corresponding author
Abstract:

The thirty one robusta accessions were analyzed for genetic diversity using RAPD molecular markers. Among the accessions, BR.11 indicated maximum dissimilarity with S.3325 (66 units) followed by S.3311 (61 units) and minimum dissimilarity was in BR.12 and L1 valley robusta (9 units). Three major clusters were observed on the dendrogram with a maximum SED of 63 units among the accessions of first cluster, 58 units in second cluster and lowest of 46 units in third cluster. The first cluster accessions showed morpho-physiologically low root biomass, root to shoot ratio and moderately high total biomass. The second cluster accessions were with reasonably high root biomass and high root to shoot ratio. The third cluster accessions belong to high root and total biomass and water use efficiency types. The Principal Component Analysis (PCA) indicated genetic variation exists between the accessions. The accessions showed with over lapping and little tight clustering in three dimensional PCA revealed relatively low genetic diversity in Indian robusta gene pool. The contrast accessions i.e. S.3334 and L1 valley robusta with respect to root, WUE and associated physiological traits were placed apart in PCAs and differed by 138 SED units and these accessions could be used to raise the mapping population in breeding programme.


Keywords: Coffea canephora, Genetic diversity, Molecular markers, Morpho-physiological traits, Cluster analysis, Principal component analysis
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How to cite this article:

Mallikarjun G. Awati, B.S. Tambat, G.F. D’souza, D. Venkataramanan, M. Udaya Kumar, C.G. Anand and Raghuramulu, Y. 2018. Assessing Genetic Diversity using RAPD Molecular markers in Coffea canephora Pierre ex. Froehner (Robusta coffee): A Step towards Crop Improvement.Int.J.Curr.Microbiol.App.Sci. 7(12): 1704-1714. doi: https://doi.org/10.20546/ijcmas.2018.712.198