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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(12): 1547-1554
DOI: https://doi.org/10.20546/ijcmas.2018.712.182


Detection of Avibacterium paragallinarum in Poultry Carcass
Sandeep Dwivedi1*, Madhu Swamy1 and Ajit Pratap Singh2
1Department of Veterinary Pathology, College of Veterinary Science and A.H., Nanaji Deshmukh Veterinary Science University, Jabalpur (M.P.) 482001
2Animal Biotechnology Center, Nanaji Deshmukh Veterinary Science University, Jabalpur (M.P.) 482001, India
*Corresponding author
Abstract:

The present study was carried out for the detection of Avibacterium paragallinarum from Avian Infectious Coryza suspected poultry carcasses of Jabalpur region. The polymerase chain reaction (PCR) was standardized for the diagnosis of Infectious Coryza by using live Avibacterium paragallinarum broth containing Modesto, Spross and 0083 strains received from Stallen South Asia Private Limited, Mumbai, as source of DNA of A. paragallinarum. Poultry carcasses (n=60) showing characteristic gross lesions of Avian Infectious Coryza were investigated in the present study. Samples were collected from the infra orbital sinus, nasal cavity and trachea of the entire sixty carcass. The swabs of the three different sites from one bird were mixed and then pooled sixty samples were tested by PCR using oligo primer specific to 16S rRNA of Avibacterium paragallinarum. PCR analysis revealed 08 positives (13.33%) for Avian Infectious coryza out of total 60 samples tested. It is concluded that PCR an early, rapid, accurate and highly sensitive diagnostic technique with the ability to substitute conventional cultural examination can be used to prevent the economic losses associated with the disease.


Keywords: Infectious coryza, Polymerase chain reaction, Poultry
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How to cite this article:

Sandeep Dwivedi, Madhu Swamy and Ajit Pratap Singh. 2018. Detection of Avibacterium paragallinarum in Poultry Carcass.Int.J.Curr.Microbiol.App.Sci. 7(12): 1547-1554. doi: https://doi.org/10.20546/ijcmas.2018.712.182