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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(12): 1160-1163
DOI: https://doi.org/10.20546/ijcmas.2018.712.143


Tilling: A Tool of Allele Mining in Plants
Suresh1*, Manjeet1 and Deepak Verma2
1Department of Genetics & Plant Breeding,
2Department of Zoology& Aquaculture,CCS Haryana Agricultural University, Hisar (Haryana), India – 125 004
*Corresponding author
Abstract:

Identification of alleles of a gene and then utilizing them in crop improvement programme is the main objective of any plant breeder. A breeding programme cannot exist if there is no variability. For the screening of germplasm for a desired allele, breeders need large area, labour and fund. Also this screening is amenable to environmental effect. Target Induced Local Lesions In Genome (TILLING) is a perfect solution of these problem. TILLING is a reverse genetics approach which uses mutagens to create noval variability in a particular allele. This variability in a gene is then screened using various sequencers to find out various mutants. The best mutant of them is then can be used in breeding programmes directly. In a modified version, natural population is screened to identify all alleles of a gene. This new version of this technique is called EcoTILLING. Using these reverse genetics approaches, a large number of genes are identified and used in breeding of various crop species.


Keywords: TILLING, EcoTILLING, Allele mining
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How to cite this article:

Suresh, Manjeet and Deepak Verma. 2018. Tilling: A Tool of Allele Mining in Plants.Int.J.Curr.Microbiol.App.Sci. 7(12): 1160-1163. doi: https://doi.org/10.20546/ijcmas.2018.712.143