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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(2): 254-262
DOI: http://dx.doi.org/10.20546/ijcmas.2016.502.028


Production and Characterization of Exopolysaccharide produced By Oil Emulsifying Bacteria
Sheetal Sonawdekar and Arpita Gupte*
School of Biotechnology and Bioinformatics,D Y Patil University, Navi Mumbai,Sector 15, Plot 50, CBD Belapur, Navi Mumbai-400 614, India
*Corresponding author
Abstract:

Exopolysaccharides (EPS) are high molecular weight polymers consisting of sugar residues which widely vary in structure and function. Many bacteria possess the ability to synthesize and excrete exopolysaccharides. In the present study hundred and twenty bacterial isolates were isolated from oil contaminated sites from in and around Navi Mumbai and Thane districts of Maharashtra. Out of which 19 different isolates were selected to explore the EPS production. These isolates were screened for oil emulsification capacity using Emulsification Index 24 test. The isolates were further tested for EPS production and optimization. The optimization was done for different time durations (24h to 1 week), nitrogen sources (Ammonium nitrate, Sodium nitrate and Ammonium chloride) and pH. Culture 1 with the best EPS producing capacity was identified as Bacillus cereus spp.The culture synthesized 520µg/ml of EPS using 0.5% Sodium nitrate at pH 7 in 48h. The FT-IR study revealed the major bands corresponding to O-H, carboxylic acid and H- bonded groups along with stretching of C-O, alcohol, ether and phenol groups. The presence of EPS on the cell surface was confirmed using Scanning Electron Microscopy.


Keywords: Exopoly-saccharides, Phenol sulfuric acid method, Bacillus cereus spp, EI 24.
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How to cite this article:

Sheetal Sonawdekar and Arpita Gupte. 2016. Production and Characterization of Exopolysaccharide produced By Oil Emulsifying Bacteria.Int.J.Curr.Microbiol.App.Sci. 5(2): 254-262. doi: http://dx.doi.org/10.20546/ijcmas.2016.502.028