National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present study was conducted to study the prevalence of the food borne zoonotic pathogen of animal origin, L. monocytogenes by isolation and identification, molecular detection and antibiotic sensitivity pattern from different samples of cattle sources in Aizawl district of Mizoram. A total 200 numbers of sample including cattle faeces (50), raw milk (50) and milk products (100) were collected randomly from different unorganized shop and farms. The seasonal variation in the occurrence of L. monocytogenes was also studied. The L. monocytogenes was isolated by using two step enrichment method of culturing and identified based on cultural characteristics, gram staining, biochemical properties, tumbling motility and in vitro pathogenicity tests. The molecular detection of L. monocytogenes strains were done by PCR using published primers. The antibiotic sensitivity was studied against 12 numbers of commonly used antibiotics in animals and human. The prevalence of L. monocytogenes was recorded as 6.50 percent including 8.00 percent from cattle faeces, 6.00 percent from raw milk, 8.00 percent from lassi, dahi and ice-cream samples, respectively. The L. monocytogenes strains showed 100 percent sensitivity towards Penicillin, Ampicillin, Oxacillin, Cephotaxime/Clavulanic acid, Ciprofloxacin, Tetracycline and Trimethoprim/Sulphamethoxazole followed by Streptomycin (84.61%), Chloramphenicol (53.84%), Gentamicin (53.84%) and Ceftriaxone (46.15%).
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