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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(11): 2829-2843
DOI: https://doi.org/10.20546/ijcmas.2018.711.326


Conventional and Molecular Detection of Listeria monocytogenes and its Antibiotic Sensitivity Profile from Cattle Sources of Aizawl, Mizoram (India)
Papia Biswas1*, Devajani Deka1, T.K. Dutta2, E. Motina1 and P. Roychoudhury2
1Department of Veterinary Public Health & Epidemiology
2Department of Veterinary Microbiology, College of Veterinary Sciences & AH, Central Agricultural University, Selesih, Aizawl, Mizoram, 796014, India
*Corresponding author
Abstract:

The present study was conducted to study the prevalence of the food borne zoonotic pathogen of animal origin, L. monocytogenes by isolation and identification, molecular detection and antibiotic sensitivity pattern from different samples of cattle sources in Aizawl district of Mizoram. A total 200 numbers of sample including cattle faeces (50), raw milk (50) and milk products (100) were collected randomly from different unorganized shop and farms. The seasonal variation in the occurrence of L. monocytogenes was also studied. The L. monocytogenes was isolated by using two step enrichment method of culturing and identified based on cultural characteristics, gram staining, biochemical properties, tumbling motility and in vitro pathogenicity tests. The molecular detection of L. monocytogenes strains were done by PCR using published primers. The antibiotic sensitivity was studied against 12 numbers of commonly used antibiotics in animals and human. The prevalence of L. monocytogenes was recorded as 6.50 percent including 8.00 percent from cattle faeces, 6.00 percent from raw milk, 8.00 percent from lassi, dahi and ice-cream samples, respectively. The L. monocytogenes strains showed 100 percent sensitivity towards Penicillin, Ampicillin, Oxacillin, Cephotaxime/Clavulanic acid, Ciprofloxacin, Tetracycline and Trimethoprim/Sulphamethoxazole followed by Streptomycin (84.61%), Chloramphenicol (53.84%), Gentamicin (53.84%) and Ceftriaxone (46.15%).


Keywords: Listeria monocytogenes, PCR, Antibiotic sensitivity, Aizawl, Mizoram
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How to cite this article:

Papia Biswas, Devajani Deka, T.K. Dutta, E. Motina and Roychoudhury, P. 2018. Conventional and Molecular Detection of Listeria monocytogenes and its Antibiotic Sensitivity Profile from Cattle Sources of Aizawl, Mizoram (India).Int.J.Curr.Microbiol.App.Sci. 7(11): 2829-2843. doi: https://doi.org/10.20546/ijcmas.2018.711.326