International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 5, Issue:2, February, 2016

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(2): 225-235

Evaluation of Phenolics Content and Invitro Antioxidant Activities of Methanolic extract of Amoora rohituka Bark
K. Umesh, C.B. Sanjeevkumar and Ramesh L Londonkar*
Department of P.G. Studies and Research in BioTechnology, Gulbarga University, Kalaburagi-585106, Karnataka, India
*Corresponding author

Numerous oxidative stress related diseases are gaining importance as a result of accumulation of free radicals in the body. A lot of researches are going on worldwide aimed towards finding natural antioxidants of plants origins. The aims of this study were to evaluate phenolic content and in vitro antioxidant activities of the methanolic extract of Amoora rohituka bark. The antioxidant and free radical scavenging activity of methanolic extract of bark were assessed against 2,2-diphenyl-1 picrylhydrazyl (DPPH), 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), Hydrogen peroxide (H2O2), ferric reducing, Phosphomolybdenum assay and metal chelation spectroscopically and results were compared with that of Butylated hydroxyl toluene (BHT) and Ascorbic acid and EDTA as standards. The IC50 value of the DPPH, ABTS, H2O2 and metal chelation are 73μg/ml, 73μg/ml, 103 μg/ml and 101μg/ml respectively. Our findings offer confirmation that the crude methanol extract of A.rohituka is a potential source of natural antioxidants, and this justified its uses in folkloric medicines.

Keywords: Amoora rohituka, Antioxidants, ABTS, DPPH, H2O2, Ferric reducing, Phospho-molybdenum,
Metal chelation

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How to cite this article:

M. K. Umesh, C.B. Sanjeevkumar and Ramesh L Londonkar. 2016. Evaluation of Phenolics Content and Invitro Antioxidant Activities of Methanolic extract of Amoora rohituka Bark.Int.J.Curr.Microbiol.App.Sci. 5(2): 225-235. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.