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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(2): 225-235
DOI: http://dx.doi.org/10.20546/ijcmas.2016.502.025


Evaluation of Phenolics Content and Invitro Antioxidant Activities of Methanolic extract of Amoora rohituka Bark
K. Umesh, C.B. Sanjeevkumar and Ramesh L Londonkar*
Department of P.G. Studies and Research in BioTechnology, Gulbarga University, Kalaburagi-585106, Karnataka, India
*Corresponding author
Abstract:

Numerous oxidative stress related diseases are gaining importance as a result of accumulation of free radicals in the body. A lot of researches are going on worldwide aimed towards finding natural antioxidants of plants origins. The aims of this study were to evaluate phenolic content and in vitro antioxidant activities of the methanolic extract of Amoora rohituka bark. The antioxidant and free radical scavenging activity of methanolic extract of bark were assessed against 2,2-diphenyl-1 picrylhydrazyl (DPPH), 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), Hydrogen peroxide (H2O2), ferric reducing, Phosphomolybdenum assay and metal chelation spectroscopically and results were compared with that of Butylated hydroxyl toluene (BHT) and Ascorbic acid and EDTA as standards. The IC50 value of the DPPH, ABTS, H2O2 and metal chelation are 73μg/ml, 73μg/ml, 103 μg/ml and 101μg/ml respectively. Our findings offer confirmation that the crude methanol extract of A.rohituka is a potential source of natural antioxidants, and this justified its uses in folkloric medicines.


Keywords: Amoora rohituka, Antioxidants, ABTS, DPPH, H2O2, Ferric reducing, Phospho-molybdenum,
Metal chelation
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How to cite this article:

M. K. Umesh, C.B. Sanjeevkumar and Ramesh L Londonkar. 2016. Evaluation of Phenolics Content and Invitro Antioxidant Activities of Methanolic extract of Amoora rohituka Bark.Int.J.Curr.Microbiol.App.Sci. 5(2): 225-235. doi: http://dx.doi.org/10.20546/ijcmas.2016.502.025