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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here
journals IJCMAS
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(11): 2229-2234
DOI: https://doi.org/10.20546/ijcmas.2018.711.249


Detection of Bovine Herpesvirus-1 Infection in Bovine Clinical Samples by Direct Fluorescent Antibody Test
Dharmesh R. Patel*, Irsadullakhan H. Kalyani, Bhavesh J. Trangadia, Kishan K. Sharma, Pushpa M. Makwana, Dhruv Desai, Dushyant Muglikar and Pramod S. Sakhare
Department of Veterinary Microbiology, Vanbandhu College of Veterinary Science and Animal Husbandry, Navsari Agricultural University, Navsari – 396 450, Gujarat, India
*Corresponding author
Abstract:

Detection of viral antigen by direct fluorescent antibody test (FAT) from clinical samples collected from cattle and buffaloes of Navsari, Surat and Valsad Districts of Southern Gujarat were carried out. Out of a total of 116 clinical samples (44-cattle, 72-buffaloes) tested, fluorescence was observed in 14 (12.09%) samples. Species-wise, eight cattle (11.11%) and 6 buffaloes (13.64%) were found to be positive by the test. The highest number of animals were detected from Surat (14.29%) followed by Valsad (12.07%) and least in Navsari (10.81%) district. Viral antigen detected in 8.00% (4/50), 7.14% (1/14), 28.57% (2/7) in cattle and 16.67%, (2/12), 16.67% (1/6), 11.547% (3/26) in buffaloes from nasal, conjunctival and vaginal swabs, respectively. Only placental cotyledon sample collected from cattle showed positive reaction by direct FAT.


Keywords: BoHV-1, Bovine, FAT, IBR
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How to cite this article:

Dharmesh R. Patel, Irsadullakhan H. Kalyani, Bhavesh J. Trangadia, Kishan K. Sharma, Pushpa M. Makwana, Dhruv Desai, Dushyant Muglikar and Pramod S. Sakhare. 2018. Detection of Bovine Herpesvirus-1 Infection in Bovine Clinical Samples by Direct Fluorescent Antibody Test.Int.J.Curr.Microbiol.App.Sci. 7(11): 2229-2234. doi: https://doi.org/10.20546/ijcmas.2018.711.249