National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present study was taken up in the Agricultural College, Bapatla during 2016-2017. Cercospora infected leaves were collected during kharif 2016 from eight different okra growing villages in Guntur district, Andhra Pradesh which were used for in planta isolation of fungal DNA and amplified using universal primers ITS 1 and ITS 4. The 550 bp amplicon thus obtained was restricted with hexa cutters, EcoRI, BamHI and tetra cutter Taq1 to find variability among Cercospora isolates. EcoRI found two restriction sites in all isolates except the Yazali isolate while BamHI found single restriction site in all the isolates with length polymorphism in Yazali isolate. Taq1 restriction indicated a high degree of genetic diversity among the isolates and was represented by three different banding patterns while in three isolates there were no restriction sites. Dendrogram constructed from similarity coefficients showed that Yazali isolate separated into a group upon digestion with EcoRI and BamHI while the Taq1 digestion of Yazali isolate clustered with Thimmareddipalem isolate. The results revealed that the presence of notable genetic variation in population sampled within the geographic region of Guntur district may be due to variations in single nucleotide polymorphism.
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