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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(11): 1720-1728
DOI: https://doi.org/10.20546/ijcmas.2018.711.197


Isolation and Identification of Enterococcus Species from Various Clinical Samples and Their Antimicrobial Susceptibility Pattern at a Tertiary Health Care Hospital in Agra
Vikas Kumar1, Astha1*, Arti Agrawal1, Yatendra Chahar1 and Naman Shree2
1Sarojini Naidu Medical College, Agra, India
2School of Life Sciences, Agra, India
*Corresponding author
Abstract:

The present study was conducted on various clinical samples, in the Department of Microbiology, S.N medical college, Agra from February 2018 to July 2018. 72 Enterococcus isolates were identified. Maximum samples from which Enterococcus spp. was isolated is urine i.e. 39 (54.17%), followed by pus and blood i.e. 22 (30.55%) and 5 (6.94%) respectively. Maximum isolate is E. faecalis i.e. 62 (86.11%) followed by E.faecium i.e. 10 (13.89%). On Antimicrobial Susceptibility testing, Ampicillin, Ciprofloxacin, Levofloxacin, Tetracycline and Doxycycline show 70.83%, 38.88%, 30.55%, 58.33% and 52.77% resistance respectively. Among the 33 Enterococcal isolates tested by disc diffusion method, Erythromycin shows 57.57% resistance. Nitrofurantoin and Norfloxacin show 15.38% and 76.92% resistance respectively, among the 39 Enterococcal isolates obtained from urine. Vancomycin shows 4.16% resistance and High Level Gentamycin shows 18.05% resistance. The emergence of Vancomycin resistance and High Level Aminoglycoside resistance in Enterococci poses a serious threat to patient’s safety as it leaves fewer options for disease management. In the present study, Teicoplanin and Linezolid were found to be 100% susceptible for all the Enterococcal isolates, hence these drugs can be prescribed even for Vancomycin Resistant Enterococci (VRE).


Keywords: Enterococcus spp., E. faecalis, E. faecium, VRE, High Level Aminoglycoside resistance
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How to cite this article:

Vikas Kumar, Astha, Arti Agrawal, Yatendra Chahar and Naman Shree. 2018. Isolation and Identification of Enterococcus Species from Various Clinical Samples and Their Antimicrobial Susceptibility Pattern at a Tertiary Health Care Hospital in Agra.Int.J.Curr.Microbiol.App.Sci. 7(11): 1720-1728. doi: https://doi.org/10.20546/ijcmas.2018.711.197