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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(11): 1692-1699
DOI: https://doi.org/10.20546/ijcmas.2018.711.193


Bio-Efficacy of Pseudomonas fluorescens Against the Root-Knot Nematode (Meloidogyne incognita) in Tomato Plant
Siddharth Shankar Sonkar1, Jayant Bhatt1, Jhumishree Meher2* and Punam Kashyap1
1Department of Plant Pathology, Jawaharlal Nehru Krishi Viswa Vidyalaya, Jabalpur, Madhya Pradesh, India
2Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India
*Corresponding author
Abstract:

Pseudomonas fluorescens culture was applied at different dilutions to induce resistance in Tomato (Solanum esculentum L.) against the root-knot nematode, Meloidogyne incognita. The efficacy of this culture, when applied as a soil drench or root dip, was compared with inoculated non-treated plants under greenhouse conditions. P. fluorescens was able to reduce nematode parameters at all dilutions and in both types of application. The dilution 9x108cfu/ml was the most effective in reducing nematode reproduction as measured by the number of developmental stages, Plant height (cm), Root length (cm), Fresh and dry weight of Shoot and Root (gm), No. of galls/plant, and No. of egg masses/gall nematode reduction was 28.68 cm, 21.83 cm, 8.90gm, 2.12gm, 4.76gm, 0.89gm, 28.75 and 38.50 respectively) when treated as a soil drench compared to the untreated control inoculated with M. incognita only. This was followed by P. fluorescens at a concentration of 6 g/kg soil which significantly reduced the same parameters by 40.30cm, 27.15cm, 35.49gm, 7.18gm, 0.85gm, 0.26gm, 17.00 and 47.75, respectively compared to control inoculated with M. incognita only. Also, plant growth criteria improved in treated plots compared to controls. The activity of three enzymes (peroxidase, polyphenol oxidase and chitinase) increased in treated plants exposed to 9x108cfu/ml as bare root dip treatment and 6 g/kg soil as soil drenching compared to the inoculated untreated control. P. fluorescens thus induced resistance in tomato against M. incognita.


Keywords: Bacterial concentration P. fluorescens, Biotic-induced resistance, Tomato, Nematode M. incognita, Bare root dip, Soil drench
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How to cite this article:

Siddharth Shankar Sonkar, Jayant Bhatt, Jhumishree Meher and Punam Kashyap. 2018. Bio-Efficacy of Pseudomonas fluorescens Against the Root-Knot Nematode (Meloidogyne incognita) in Tomato Plant.Int.J.Curr.Microbiol.App.Sci. 7(11): 1692-1699. doi: https://doi.org/10.20546/ijcmas.2018.711.193