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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
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Index Copernicus ICV 2017: 100.00
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Int.J.Curr.Microbiol.App.Sci.2018.7(11): 725-733
DOI: https://doi.org/10.20546/ijcmas.2018.711.087


In vitro Regeneration by Somatic Embryos of Maize Inbreds Using Mature Seeds
N.K. Dhillon1* and S.S. Gosal2
1Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana-141004, Punjab, India
2Punjab Agricultural University, Ludhiana-141004, Punjab, India
*Corresponding author
Abstract:

Five maize lines prevalently used by breeders in the maize program were screened for their ability to form type II callus. Mature seeds were used as explant for in vitro culturing. One inbred line showed high embryogenic response and formed friable, type II calli and also exhibited successful plant regeneration. Ten culture media compositions based on Murashige and Skoog (1962) salts thus investigated differed with respect to the combinations of concentrations of auxins (IBA, NAA, picloram, 2, 4-D) and cytokinins (BAP, kinetin) were used. Highest percent callus induction and subsequent somatic embryogenesis were observed in inbred LM13. The embryogenic calli were maintained and multiplied on regeneration medium containing reduced levels of auxins. Medium MS + Proline (3.0 gL-1) + BAP (3.0 mgL-1) + Kn (0.5 mgL-1) + Gelrite (0.2%) unsurpassed plant regeneration capacity. This efficient regeneration system provides a solid basis for genetic transformation of maize.


Keywords: Maize, Mature seed, Somatic embryogenesis, SEM
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How to cite this article:

Dhillon, N.K. and Gosal, S.S. 2018. In vitro Regeneration by Somatic Embryos of Maize Inbreds Using Mature Seeds.Int.J.Curr.Microbiol.App.Sci. 7(11): 725-733. doi: https://doi.org/10.20546/ijcmas.2018.711.087