National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Five maize lines prevalently used by breeders in the maize program were screened for their ability to form type II callus. Mature seeds were used as explant for in vitro culturing. One inbred line showed high embryogenic response and formed friable, type II calli and also exhibited successful plant regeneration. Ten culture media compositions based on Murashige and Skoog (1962) salts thus investigated differed with respect to the combinations of concentrations of auxins (IBA, NAA, picloram, 2, 4-D) and cytokinins (BAP, kinetin) were used. Highest percent callus induction and subsequent somatic embryogenesis were observed in inbred LM13. The embryogenic calli were maintained and multiplied on regeneration medium containing reduced levels of auxins. Medium MS + Proline (3.0 gL-1) + BAP (3.0 mgL-1) + Kn (0.5 mgL-1) + Gelrite (0.2%) unsurpassed plant regeneration capacity. This efficient regeneration system provides a solid basis for genetic transformation of maize.
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