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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(11): 509-515
DOI: https://doi.org/10.20546/ijcmas.2018.711.061


Mutagenic Effectiveness and Efficiency of Gamma Rays, Ethyl Methane Sulphonate and their Combination Treatments in Chickpea (Cicer arietinum L.)
Kamal Dev Sharma1*, Gopal Katna2, Neha Sharma2, Ruby Nag1, Bipan Kumar Sharma3 and Archana Joshi Saha4
1Department of Agricultural Biotechnology
2Department of Crop Improvement, CSK HP Agricultural University, Palampur – 176062, Himachal Pradesh, India
3CSK HPKV Research Sub Station, Akrot, - 177211, Himachal Pradesh, India
4Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Mumbai - 400085, Maharashtra, India
*Corresponding author
Abstract:

A systematic and comparative study on mutagenic ability of different doses of ethyl methane sulphonate (EMS, an alkylating agent), gamma rays (an ionizing radiation) and EMS + gamma rays was carried out in a well-adapted desi chickpea variety HPG17. All mutagenic treatments were effective in inducing genetic variability. A proportional decrease in germination of mutagen treated seeds as well as subsequent plant survival was observed with increase in mutagen dose. Not only higher doses but combined treatments having low doses of EMS + gamma rays also reduced seed germination and plant survival with highest reduction in seed germination at 200 Gy + 0.05% EMS and in plant survival at 150 Gy + 0.05%. EMS appears to be better than gamma rays in induction of macromutations as maximum frequency of macromutants was at EMS (0.10%). Mutagenic effectiveness and efficiency was also studied to identify the most effective mutagen treatment. Overall EMS was more effective than the gamma rays and combination treatments as maximum effectiveness was observed at 0.05% and 0.10% EMS, respectively. The 300 Gy + 0.05% EMS had the highest mutagenic efficiency.


Keywords: EMS, Gamma rays, Effectiveness, Efficiency, Induced mutations, Chickpea
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How to cite this article:

Kamal Dev Sharma, Gopal Katna, Neha Sharma, Ruby Nag, Bipan Kumar Sharma and Archana Joshi Saha. 2018. Mutagenic Effectiveness and Efficiency of Gamma Rays, Ethyl Methane Sulphonate and their Combination Treatments in Chickpea (Cicer arietinum L.).Int.J.Curr.Microbiol.App.Sci. 7(11): 509-515. doi: https://doi.org/10.20546/ijcmas.2018.711.061