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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(11): 452-460
DOI: https://doi.org/10.20546/ijcmas.2018.711.053


Evaluation of Culture Methods for Isolation of Mycobacterium tuberculosis Complex and Its Resistance among Pulmonary Tuberculosis Cases in a Tertiary Care Setting, Kerala - A Pilot Study
Sreeja Nair1, Seema Oommen1* and Vidya Pai2
1Department of Microbiology, Pushpagiri Institute of Medical Sciences and Research Centre, Tiruvalla, Kerala, India
2Department of Microbiology, Yenepoya Medical College, Yenepoya University, Mangalore, Karnataka, India
*Corresponding author
Abstract:

Tuberculosis is an important public health problem in India and globally. Prompt detection and susceptibility testing of Mycobacterium tuberculosis complex from clinical specimens is essential for appropriate management of patients with tuberculosis. With this in background, this study aimed at the comparison of isolation of Mycobacterium tuberculosis complex from clinical specimens of patients suspected of pulmonary tuberculosis using BACTEC Micro MGIT and Lowenstein Jensen (LJ) media with detection of resistance. A prospective study was carried out for one year from November 2016 to October 2017 in the Department of Microbiology, Pushpagiri Medical College, Tiruvalla, Kerala. A total of 81 samples were processed for Ziehl-Neelsen (Z-N) staining and was then cultured using liquid Mycobacterium growth indicator tube (BDTM BACTECTM Micro MGIT) and solid (Lowenstein Jensen’s) media. Isolates confirmed as Mycobacterium tuberculosis complex were subjected to drug susceptibility testing (DST) using modified proportion method by MGIT for 1st line drugs i.e. streptomycin (1.0µg/ml), isoniazid (0.1µg/ml), rifampicin (1µg/ml), ethambutol (5µg/ml) and pyrazinamide (100µg/ml). Of the total 81 samples that were cultured, seven (8.6%), were positive by both MGIT and Lowenstein Jensen’s. Three (3.7%) were positive by MGIT only and not by LJ. Only 4/10 (4.9%) culture positive samples were positive by direct smear examination. Turnaround times for culture positivity by MGIT vs LJ was an average of 15.1 days vs 23.8 days. Sensitivity to all drugs was observed in 4/10 (40%) isolates. None of these 10 isolates were multidrug resistant. Culturing of the organism in addition to smear, increases the possibility of microbiological diagnosis and offers a possibility for drug susceptibility testing.


Keywords: Pulmonary tuberculosis (PTB), Mycobacterium tuberculosis complex (MTB complex), Mycobacterium growth indicator tube (MGIT), Lowenstein Jensen (LJ), Drug susceptibility testing (DST)
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How to cite this article:

Sreeja Nair, Seema Oommen and Vidya Pai. 2018. Evaluation of Culture Methods for Isolation of Mycobacterium tuberculosis Complex and Its Resistance among Pulmonary Tuberculosis Cases in a Tertiary Care Setting, Kerala - A Pilot Study.Int.J.Curr.Microbiol.App.Sci. 7(11): 452-460. doi: https://doi.org/10.20546/ijcmas.2018.711.053