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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(11): 351-362
DOI: https://doi.org/10.20546/ijcmas.2018.711.044


Analysis of Sera from Cattle Suspected for Brucellosis by Agglutination Tests and ELISA
Anju Mohan and Hari Mohan Saxena*
Department of Veterinary Microbiology, College of Veterinary Science, GADVASU, Ludhiana-141004, Punjab, India
*Corresponding author
Abstract:

Brucellosis is a major bacterial zoonosis of global importance. Bovine brucellosis is endemic in all states of India. However, the exact extent of the disease occurrence in the unorganized herds is not known. The present study was undertaken for serological diagnosis of brucellosis in cattle from a local gaushala (cow shelter) employing common agglutination tests RBPT, STAT and MAT and ELISA. Out of 56 samples from cattle from the Gaushala, 37.5% samples by RBPT, 64.28% each by STAT and MAT and 49.23% samples by ELISA, respectively were found to be positive for anti-Brucella antibodies. Among the 29 suspected serum samples from the field cattle, samples found to be positive for anti-Brucella antibodies were 100% by RBPT, 79.31% by MAT and 89.65% by ELISA, respectively. Titers of anti-Brucella antibodies in the sera of cattle from Gaushala were found to be 1.747+0.45, 1.935+0.62 and 0.943+0.46 by STAT, MAT and ELISA, respectively, while those in cattle from the field were found to be 1.577+0.60 and 0.783+0.59 by MAT and ELISA, respectively.


Keywords: Brucellosis, RBPT, STAT, MAT and ELISA
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How to cite this article:

Anju Mohan and Hari Mohan Saxena. 2018. Analysis of Sera from Cattle Suspected for Brucellosis by Agglutination Tests and ELISA.Int.J.Curr.Microbiol.App.Sci. 7(11): 351-362. doi: https://doi.org/10.20546/ijcmas.2018.711.044