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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(11): 148-159
DOI: https://doi.org/10.20546/ijcmas.2018.711.019


Occurrence of Fusarium oxysporum (Schlecht. Emend. Snyder & Hansen) Causing Pod Blight of Soybean (Glycine max L.) and Its Suitable Management with Native Trichoderma spp. in Manipur, India
C. Lalhruaitluangi*, B. Sinha and Tusi Chakma
Department of Plant Pathology, College of Agril., CAU, Imphal-795004, Manipur, India
*Corresponding author
Abstract:

During 2015-16 survey was conducted in six different soybean growing areas of Manipur, India. The survey revealed that the maximum mean pod blight intensity was at Thoubal (21.23%) and minimum (13.82%) at Andro Research Farm, CAU. Fusarium oxysporum was isolated and identified by morphological characteristics and gene sequencing, MF-512000. The pathogen was found to be the causal organism of pod blight of soybean. Total genomic DNA of the fungal cultures was extracted by using HiPurA DNA isolation Kit (HiMedia, India). PCR amplification of internal transcribed spacer regions was done using specific ITS1 and ITS4 primers and was confirmed by 1.2% agarose gel electrophoresis which produced a fixed region length of approximately 600bp for Fusarium oxysporum. Effects of volatile compounds produced by the Trichoderma spp. against Fusarium oxysporum ranged from 26.27-42.40 per cent. The effects of non-volatile compounds produced by Trichoderma spp. ranged from 20.39-43.52 per cent at 7.5% v/v concentration and 29.41-53.72 per cent at 15% v/v. In vivo study of isolates of Trichoderma spp. under field trials as seed treatment (@ 5g/kg seed) with foliar spraying (@ 5g/l of water) at 40 days after planting of water showed considerable reduction in disease incidence and increased production over control plot. The isolate T. harzianum (KU933468) showed highest yield in field trial (17.44 q/ha).


Keywords: Fusarium oxysporum, PCR, Pod blight, Trichoderma, Soybean
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How to cite this article:

Lalhruaitluangi, C., B. Sinha and Tusi Chakma. 2018. Occurrence of Fusarium oxysporum (Schlecht. Emend. Snyder & Hansen) Causing Pod Blight of Soybean (Glycine max L.) and Its Suitable Management with Native Trichoderma spp. in Manipur, India..Int.J.Curr.Microbiol.App.Sci. 7(11): 148-159. doi: https://doi.org/10.20546/ijcmas.2018.711.019