International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 5, Issue:10, October, 2016

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(10): 1125-1129

Recognition of Dermatophytes by Dermatophyte Test Medium
Th.  Nabakumar Singh1*, G. Zamzachin2 and Ng. Brajachand Singh1
1Department of Microbiology, Regional Institute of Medical Sciences (RIMS), Imphal-795001, Manipur, India
2Department of Dermatology, Regional Institute of Medical Sciences (RIMS), Imphal-795001, Manipur, India
*Corresponding author

The purpose of the study is to determine the clinical variants, their etiological agents and to compare Sabouraud dextrose agar (SDA) and dermatophyte test medium (DTM) for primary isolation of dermatophytes from one hundred clinically suspected cases of dermatophytoses. The culture was done in SDA and DTM and dermatophytes characterization were made by slide culture, urease test and hair perforation test. Among the dermatophytes isolated, the commonest was Trichophyton rubrum (55.22%) followed by Trichophyton mentagrophytes (34.33%) and Epidermophyton floccosum (10.45%). Majority of the patients had tinea corporis (31.43%) followed by tinea cruris (19.40%).  The rate of culture positive by SDA and DTM were 65% and 67% respectively which shows no significant difference between the two media for primary isolation of dermatophytes. The present study shows DTM is good for isolation and early recognition of dermatophytes because of a distinct color change in the medium within two weeks time however, standard media such as SDA is required for species identification.

Keywords: Dermatophytes /DTM/SDA/RIMS Hospital

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How to cite this article:

Nabakumar Singh, Th., G. Zamzachin and Brajachand Singh, Ng. 2016. Recognition of Dermatophytes by Dermatophyte Test Medium.Int.J.Curr.Microbiol.App.Sci. 5(10): 1125-1129. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.