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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(10): 1114-1124
DOI: http://dx.doi.org/10.20546/ijcmas.2016.510.117


Rapid Confirmation and Molecular Identification of Alkaline Protease Producing Aspergillus awamori through Submerged Fermentation
Radhika Pilli1 and K.G. Siddalingeshwara2*
1Research and Development Centre, Bharathiar University, Coimbatore, India
2Scientific and Industrial Research Centre, Bangalore, India
*Corresponding author
Abstract:

Recent years have witnessed a phenomenal increase in the use of enzymes as industrial catalysts. Proteases constitute a very large and complex group of enzymes, widely utilized in a host of industries. Industrially important efficient alkaline protease producer was isolated from soil samples collected from different regions of Bangalore. Among the 25 isolates Aspergillus awamori KGSR 12 evolved as an potential protease producer by plate assay method and it showed 1.2 cm diameter, 46.15% hydrolysis. The protease were confirmed by TLC method by Rf value i.e 0.84.  The molecular tool used for identification of Aspergillus awamori KGSR 12 and also optimum percentage of hydrolysis (0.5%) were determined.


Keywords: Alkaline protease, Molecular confirmation, Casein and Submerged fermentation
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How to cite this article:

Radhika Pilli and K.G. Siddalingeshwara. 2016. Rapid Confirmation and Molecular Identification of Alkaline Protease Producing Aspergillus awamori through Submerged Fermentation.Int.J.Curr.Microbiol.App.Sci. 5(10): 1114-1124. doi: http://dx.doi.org/10.20546/ijcmas.2016.510.117