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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(10): 1105-1113
DOI: http://dx.doi.org/10.20546/ijcmas.2016.510.116


Isolation of L-Asparaginase Producing Microbial Strains from Soil Samples of Telangana and Andhra Pradesh States, India
A.S. Lalitha Devi* and R. Ramanjaneyulu
Department of Microbiology, Sri Krishnadevaraya University, Anantapuram, Andhra Pradesh-515003, India
*Corresponding author
Abstract:

L-Asparaginase fits to a cluster of homologous amidohydrolases which catalyses the hydrolysis of aminoacid L-asparagine to L-aspartate and ammonia. The present study deals with isolation of L-Asparaginase enzyme producing bacterial strains, through serial diluting the soil samples from different places of Telangana and Andhra Pradesh states, India. L-asparaginase activity was detected on the basis of formation of pink colour around the colony. A total of 132 colonies were screened and isolated from all the samples. Based on the zone of diameter it can be concluded as good L-asparaginase producers and poor L-asparaginase producers. This experiment is mainly to isolate L-asparaginase producing microbial strains collected from various soil samples.


Keywords: L-asparaginase, Soil samples, biochemical characterization, microbial strains
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How to cite this article:

Lalitha Devi, A.S., and Ramanjaneyulu, R. 2016. Isolation of L-Asparaginase Producing Microbial Strains from Soil Samples of Telangana and Andhra Pradesh States, India.Int.J.Curr.Microbiol.App.Sci. 5(10): 1105-1113. doi: http://dx.doi.org/10.20546/ijcmas.2016.510.116