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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(10): 1097-1104
DOI: http://dx.doi.org/10.20546/ijcmas.2016.510.115


Selection of Storage Methods for Maintenance of Different Stock Cultures
Angshumanjana*, Anirbanjana, Dipjitdey, Arijitmajumdar, Jayantabikashdey and Nikhil Tudu
IIT Campus, Khargapur, West Bengal, India
*Corresponding author
Abstract:

Now a day, culture maintenance is a big problem for quality control of microorganisms. The microbiologist needs to have some convenient methods for maintaining microorganisms. There are multiple methods for microbial preservation. Samples were collected from the patients in the department of microbiology at BSMC & H, Bankura, a tertiary care hospital, over a period of four months from March to June 2016. In this study performed four simple methods for preservation of bacteria such as 20%glycerol supplemented broth preserved at -20oC temperature, 0.4% semisolid agar media at 4oC, Nutrient agar at 4oC, Nutrient agar at 8oC. During preservation we performed biochemical test and observed antibiotic sensitivity pattern after each 2weeks interval. In20%glycerol broth at -20oC of Pseudomonas sp and Klebsiella sp were not viable after 7-8 weeks of preservation. In semisolid agar media all of 6 bacteria were aliveupto12 weeks. In nutrient agar medium at 4oC and at 8oC bacteria were alive up to 6-9 weeks but antibiotic sensitivity pattern showed marked change in change zone diameter for some bacteria. This large change of zone diameter was not due to human error so we cannot preserve for more than 6 weeks in Nutrient agar. From this above experiment we can conclude that preservation in 0.4% semisolid agar media at 4oC is the ideal media for the preservation of Gram positive and Gram negative bacteria.


Keywords: Stock culture, 20% glycerol, semisolid agar, nutrient agar, Pseudomonas sp and Klebsiella sp
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How to cite this article:

Angshumanjana, Anirbanjana, Dipjitdey, Arijitmajumdar,    Jayantabikashdey and Nikhil Tudu. 2016. Selection of Storage Methods for Maintenance of Different Stock Cultures.Int.J.Curr.Microbiol.App.Sci. 5(10): 1097-1104. doi: http://dx.doi.org/10.20546/ijcmas.2016.510.115