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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(2): 190-201
DOI: http://dx.doi.org/10.20546/ijcmas.2016.502.022


Formulation of β-lactam Antibiotic Encapsulated Micro- and Multiple-Emulsions, and Evaluation of its Antibacterial Activity against β-lactamase Producing Uropathogens
Mobashshera Tariq1, Swapnil Mohurle2, Vandana B. Patravale2 and K. Aruna1*
1Department of Microbiology, Wilson College, Mumbai 400 007, India

2Department of Pharmaceutical Science and Technology, Institute of Chemical Technology, Matunga, Mumbai 400 019, India
*Corresponding author
Abstract:

Growing incidence of β-lactam resistance mediated by Extended Spectrum β-Lactamases (ESBLs) and Metallo-β-lactamases (MBLs) is a major concern due to their extreme resistance towards the commonly used antibiotics. The present study was undertaken to determine the in-vitro effect of antibiotic encapsulated micro/multiple emulsions on clinical isolates of 68 ESBL and 7 MBL-producing uropathogens by agar diffusion method. The water in oil (w/o) microemulsions of amoxicillin (particle size 11-15nm) and ceftazidime (particle size 17-19nm) did not exhibit antibacterial activity. However, water in oil in water (w/o/w) multiple-emulsion of both the drugs exhibited activity. Further, the effect of glyceryl dilaurate (GDL), a lipid emulgent having inherent antibacterial activity was studied. Ceftazidime multiple emulsions, with and without GDL showed zones of inhibition against all test uropathogens in the range of 11-32mm. Amoxicillin w/o/w multiple-emulsion without GDL was found to be ineffective. However, in presence of GDL, comparatively better activity was observed with zones of inhibition in the range of 10-13mm. All micro/multiple emulsions showed a PDI (Poly Dispersity Index) between 0.1-0.3. This study hereby indicates the potential of antibiotic micro/multiple emulsions to be used effectively for intracellular targeting.


Keywords: β-lactam, antibacterial, ESBL,MBL, Multiple emulsions,micro emulsions, GDL
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How to cite this article:

Mobashshera Tariq, Swapnil Mohurle, Vandana B. Patravale and Aruna. K. 2016. Formulation of β-lactam Antibiotic Encapsulated Micro- and Multiple-Emulsions, and Evaluation of its Antibacterial Activity against β-lactamase Producing Uropathogens.Int.J.Curr.Microbiol.App.Sci. 5(2): 190-201. doi: http://dx.doi.org/10.20546/ijcmas.2016.502.022