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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(10): 2452-2461
DOI: https://doi.org/10.20546/ijcmas.2018.710.284


Cloning and Expression of Recombinant VP2 Capsid Protein Gene of Canine Parvovirus in E. coli System
Sangeetha Subramani1, Hirak Kumar Mukhopadhyay1, Mouttou Vivek Srinivas1, Muthuraj Muthaiah3, Prabhakar Xavier Antony1 and Jacob Thanislass2
1Department of Veterinary Microbiology
2Department of Veterinary Biochemistry, Rajiv Gandhi Institute of Veterinary Education & Research, Puducherry - 605 009, India
3Microbiology Laboratory, TB and Chest Disease Hospital, Puducherry - 605 006, India
*Corresponding author
Abstract:

Canine parvovirus type - 2 (CPV-2) infection is one of the most important viral diseases in dogs and wild carnivores causing severe haemorrhagic gastroenteritis in young ones. VP2 capsid protein plays an important role in determining the antigenicity and diversity of the virus. Although, several CPV variants emerged but new CPV-2a is the predominant circulating field strain of CPV in India. In the present study, new CPV-2a field strain (KLD3) isolated in cell culture was selected and the whole CPV VP2 gene was used for the expression in the E. coli expression system. Prokaryote expressing monocistronic DNA cassette containing open reading frame of whole CPV capsid gene (VP2) downstream of T7 promoter was synthesized. Analysis of the expression in E. coli cells showed the presence of capsid protein. Recombinant capsid protein showed immunoreactivity similar to the whole CPV virus antigen, when reacted with polyclonal antibodies against the whole CPV virus particles. The use of indigenously developed recombinant protein, being very economical, can be used to develop field kit. As the recombinant protein is not infectious, use of it for CPV serodiagnostic assay is considered safe.


Keywords: Canine parvovirus type - 2 (CPV-2), E. coli, CPV
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How to cite this article:

Sangeetha Subramani, Hirak Kumar Mukhopadhyay, Mouttou Vivek Srinivas, Muthuraj Muthaiah, Prabhakar Xavier Antony and Jacob Thanislass. 2018. Cloning and Expression of Recombinant VP2 Capsid Protein Gene of Canine Parvovirus in E. coli System.Int.J.Curr.Microbiol.App.Sci. 7(10): 2452-2461. doi: https://doi.org/10.20546/ijcmas.2018.710.284