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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(10): 931-937
DOI: http://dx.doi.org/10.20546/ijcmas.2016.510.100


Comparative Analysis of Rapid Dengue Testing and ELISA for NS1 Antigen and IgM in Acute Dengue Infection
Mahesh Reddy*, Kavita Sahai, Ajay Malik, S. Shoba and Anurag Khera
Department of Microbiology, Armed Forces Medical College Pune – 411048, Maharashtra, India
*Corresponding author
Abstract:

Dengue cases in India are increasing in epidemic proportions. The diagnosis of acute dengue infection is made by viral isolation and identification, viral nucleic acid detection, and serological tests for NS1 Antigen or IgM or IgG seroconversion. Viral isolation, viral nucleic acid detection requires specialized laboratory equipment and expertise. For resource limited labs tests like rapid immunochromatography (ICT) based or ELISA based tests for detection of NS1 antigen or IgM antibody can be used. In this present study we attempted to do a diagnostic test evaluation of rapid ICT with ELISA for detection of NS1 antigen (in 5 days fever)for acute dengue diagnosis. The serum samples from the first group (n=740) were subjected to NS1 antigen detection by rapid and ELISA and that of the second group (n=609) were subjected to IgM detection by rapid and IgM capture ELISA. The sensitivity of the rapid ICT tests for NS1 Ag & IgM in our study was more than 90 %, and specificity more than 98%.We noted that rapid ICT kits that we used for testing NS1Ag and IgM performed at par with ELISA based tests.


Keywords: Acute dengue infection, NS1 antigen, IgM antibody, rapid immunochromatography, MAC ELISA.
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How to cite this article:

Mahesh Reddy, R., Kavita Sahai, Ajay Mallik, S. Shoba and Anurag Khera. 2016. Comparative Analysis of Rapid Dengue Testing and ELISA for NS1 Antigen and IgM in Acute Dengue Infection.Int.J.Curr.Microbiol.App.Sci. 5(10): 931-937. doi: http://dx.doi.org/10.20546/ijcmas.2016.510.100