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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(10): 2225-2231
DOI: https://doi.org/10.20546/ijcmas.2018.710.255


Molecular Prevalence of Theileria annulata in Cattle from Different Agroclimatic Zones of Tamil Nadu, India
R. Edith1*, T.J. Harikrishnan1, G. Ponnudurai4, S. Gomathinayagam1, P. Kumarasamy2 and T.M.A. Senthilkumar3
1Department of Veterinary Parasitology
2Department of Animal Genetics and Breeding
3Department of Animal Biotechnology, Madras Veterinary College, Chennai-600 007, Tamil Nadu, India
4Department of Veterinary Parasitology, Veterinary College and Research Institute, Namakkal-637 402, Tamil Nadu Veterinary and Animal Sciences University, India
*Corresponding author
Abstract:

Bovine tropical theileriosis caused by Theileria annulata is an economically important disease of cattle in tropical and subtropical countries. Conventional diagnostic methods are unable to identify the subclinical and carrier status of this disease. Molecular diagnostic methods are more sensitive in detection of subclinical and carrier condition on the disease. In this study 817 blood samples were collected from cattle of seven different agroclimatic zone of Tamil Nadu state. DNA extracted from these blood samples were screened by nested Polymerase Chain reaction using primers targeting the partial region of 18SrRNA gene of T. annulata. 114 samples were positive out of 817 samples. North eastern zone showed more prevalence (20.57 %) followed by North western zone (18.80%) whereas hilly zone (3.23%) showed least prevalence of T. annulata in cattle. Present study shows that the 18S rRNA based molecular screening of T. annulata infection in cattle is useful to assess the prevalence of bovine tropical theileriosis.


Keywords: Cattle, Molecular prevalence, Theileria annulata, 18S rRNA, Agroclimatic zones, Nested PCR, Tamil Nadu, India
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How to cite this article:

Edith, R., T.J. Harikrishnan, G. Ponnudurai, S. Gomathinayagam, P. Kumarasamy and Senthilkumar, T.M. A. 2018. Molecular Prevalence of Theileria annulata in Cattle from Different Agroclimatic Zones of Tamil Nadu, India.Int.J.Curr.Microbiol.App.Sci. 7(10): 2225-2231. doi: https://doi.org/10.20546/ijcmas.2018.710.255