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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(10): 1939-1944
DOI: https://doi.org/10.20546/ijcmas.2018.710.223


Evaluation of Bacillus - Calmette- Guerin (BCG) Immunogenicity in Indigenous Calves under Field Condition
Asokkumar1, G. Selvaraju1, S. Manoharan2, K.G. Tirumurugaan3, V. Maroudam4 and M. Vijayabharathi1
1Department of Veterinary Preventive Medicine, Madras Veterinary College, TANUVAS, Chennai –7, Tamil Nadu, India-2Vaccine Research Centre – Bacterial Vaccines, TANUVAS, Chennai-51, Tamil Nadu, India
3Department of Animal Biotechnology, Madras Veterinary College, TANUVAS, Chennai –7, Tamil Nadu, India
4(Penn. State University, USA and Melinda Gates Foundation), Animal TB control Project, TANUVAS, Chennai-600 051, Tamil Nadu, India
*Corresponding author
Abstract:

Bovine tuberculosis (bTB) remains a major problem causing huge economic loss in livestock farming. It can spread to humans from infected cattle either through aerosol or by consumption of contaminated dairy products to cause zoonotic tuberculosis. Current Indian diagnostic approach is by intra dermal injection of PPD (tuberculin skin test) derived from the Mycobacterium bovis (bovine PPD) wherein the infected animals provokes a measurable skin reaction. BCG vaccination is not used to control bovine tuberculosis because of minimal data available on its vaccine immunogenicity and efficacy studies in cattle. Thus it’s important to test the BCG vaccine performance in the Indian cattle population. The present study aimed at evaluating the BCG vaccine immunogenicity testing in young sahiwal calves. The bTB screening and vaccine immunogenicity were evaluated using the OIE approved Interferon gamma release assay (IGRA) using BOVIGAM™ kit. BCG was titrated with 105 colony forming units (CFU) and observed from the present study that low dose of 10(CFU) is found to be effective for inducing significant bTB specific protective IFN-gamma at day 14 post vaccination. Thus the conventional BCG vaccination shall be considered as an approach for the control as well as reducing the bio burden of bovine tuberculosis along with complementary diagnosis.


Keywords: Bovine Tuberculosis- BCG – Immune Response – Indigenous Calves
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How to cite this article:

Asokkumar, M., G. Selvaraju, S. Manoharan, K.G. Tirumurugaan, V. Maroudam and Vijayabharathi, M. 2018. Evaluation of Bacillus - Calmette- Guerin (BCG) Immunogenicity in Indigenous Calves under Field Condition.Int.J.Curr.Microbiol.App.Sci. 7(10): 1939-1944. doi: https://doi.org/10.20546/ijcmas.2018.710.223