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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(10): 1469-1476
DOI: https://doi.org/10.20546/ijcmas.2018.710.164


Molecular and Phenotypic Characterization for Leaf Rust Resistance in Bread Wheat (Triticum aestivum L.)
Arati Yadawad*, R.R. Hanchinal, H.L. Nadaf, S.A. Desai, Suma Biradar and V. Rudranaik
Department of Genetics and Plant Breeding, University of Agricultural Sciences, Dharwad-58000, Karnataka, India
*Corresponding author
Abstract:

Present study aimed at molecular and phenotypic characterization of F3 population of a cross between leaf rust susceptible but well adapted bread wheat variety DWR 162 and resistant variety PBW 343 carrying highly effective alien genes Lr24 and Lr28 to identify recombinant lines with high productivity and leaf rust resistance. Fifty nine F3 lines with seven checks were evaluated for yield and yield contributing traits in randomized block design (RBD) with two replications. These lines were also screened against most virulent pathotypes of leaf rust 77-5 (121R63-1) in rust screening nursery. SCAR markers, namely SCS1302607 and SCS421570 linked to Lr24 and Lr28 genes facilitated identification of individual lines possessing the targeted genes. To identify the lines that mostly resemble ‘DWR 162’ variety, DUS (Distinctiveness Uniformity Stability) characterization was done. Nine lines were identified in F3 generation, possessing high yield potential and leaf rust resistance genes, Lr24 and Lr28 with the desirable features of DWR 162. Availability of a combination of two major rust resistance genes would facilitate the breeding program for developing new leaf rust resistant and high yielding cultivars.


Keywords: Marker assisted selection, Leaf rust, Resistance genes, Triticum aestivum
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How to cite this article:

Arati Yadawad, R.R. Hanchinal, H.L. Nadaf, S.A. Desai, Suma Biradar and Rudranaik, V. 2018. Molecular and Phenotypic Characterization for Leaf Rust Resistance in Bread Wheat (Triticum aestivum L.).Int.J.Curr.Microbiol.App.Sci. 7(10): 1469-1476. doi: https://doi.org/10.20546/ijcmas.2018.710.164