International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 5, Issue:10, October, 2016

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(10): 778-782

Hepatitis B Surface Antigen Detection by Enhanced hemiluminiscence A Pilot Study to Determine the Gray Zone Results
Ryhan Bashir1*, M.H. Sharif2, Vidhya1 and Varsha Saxena1
Department of Microbiology, YMCH, Mangalore, India
Department of Pathology, YMCH, Mangalore, India  
*Corresponding author

The hepatitis B virus infection is a worldwide serious clinical hitch because of its potential adverse sequelae. Detection of HBsAg can be done by different methods like Immunochromatographic (ICT) and fully automated chemiluminiscence immunoassays (CLIA). This study was undertaken to detect the presence of hepatitis B virus surface antigen (HBsAg) by Immunochromatographic test method (ICT) and eCLIA, compare them and to check the accuracy of eCLIA. 9842 serum specimens were subjected to HBsAg detection by Immunochromatographic assay (ICT) and enhanced chemiluminiscence immunoassay (eCLIA). The specimens that showed COI values between 0.9 and 35.0 by eCLIA testing were subjected to confirmatory testing using Vitros Immunodiagnostics HBsAg confirmatory kit.  Out of 9842 serum specimens tested40were found reactive. Among these 28 were found reactive by both ICT and eCLIA and 12 by only eCLIA. The confirmatory test that was carried out on 12 samples showed only 2 specimens with COI> 25.4 were true positive. ROC curve analysis of eCLIA showed an area of 0.8338. The eCLIA is an accurate and good test method for the detection of HBsAg. However, weakly reactive results need to be confirmed by confirmatory test methods to rule out false positives.

Keywords: HBsAg,eCLIA,Immuno-chromatographic ICT.

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How to cite this article:

Ryhan Bashir, M.H. Sharif, Vidhya and Varsha Saxena. 2016. Hepatitis B Surface Antigen Detection by Enhanced Chemiluminiscence-A Pilot Study to Determine the Gray Zone Results.Int.J.Curr.Microbiol.App.Sci. 5(10): 778-782. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.