International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 5, Issue:10, October, 2016

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(10): 708-717

Isolation of Tomato Fruits Mycoflora and Evaluation in vitro and in vivo by Trichoderma harzianum
Mokhtar Hamitou1,2*, Laid Dehimat2 and M. Mourad Senoussi1
1Laboratory of Bimolecular and Plant Amelioration, Larbi-Ben-M’hidi University, Oum El Bouaghi, BP 358, Constantine Road, 04000, Algeria
2Laboratory of Mycology, Biotechnology and Microbial activity, University of Mentouri, Constantine, 25000. Algeria
*Corresponding author

The present investigation aimed is to isolate and identify the mycetes accompanying the tomato fruits (Lycopersicon esculentum), and to evaluate    in vitro and in vivo the ability of T.harzianum to control the isolated mycetes. Some infected tomato fruits by mycetes were brought from Oum-elbouaghi market. The results of isolation allowed the identification of Stemphylium sp. and Aspergillus niger. One isolate of T.harzianum / Hypocrea lixii was utilized in this study.  The results of direct confrontation (in vitro) of T.harzianum against Stemphylium sp. and A.niger on PDA medium indicated the inhibition of mycelium growth in variable degrees; it was equal in the fourth day of the experiment to 54.54 % and 52.17%   for Stemphylium sp. and A.niger respectively. However, it did not show any growth of the tested fungus when re-planting a disk from the interaction hyphal area between T.harzianum and Stemphylium sp. or A.niger from dual cultures, while T.harzianum grew alone in the plates. The microscopic observations of mycelia of dual culture  in slide methods showed that the mycelia  of  T.harzianum induced degradation and aggregated the  spores and  analyzed the mycelia of A.niger , overgrowing  the mycelia of Stemphylium sp  and coiled around of them and  degrading  them. In vivo screening showed after 10 days of incubation an antagonistic activity of T.harzianum against the tested fungus on tomato fruits, with inhibition equal to    100 % and 95% in Stemphylium sp. and in A.niger respectively, compared with controls. Beside we found that the treated fruits with T.harzianum stayed saints as compared with control, when Stemphylium  and A.niger soft rot infected all surface the test fruits. This strain of T.harzianum may offer potential for biological control of tomato Stemphylium and A.niger soft rot.

Keywords: Stemphylium sp., soft rot,Aspergillus nigerLycopersicon esculentum, confrontation,slide methods,inhibition

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Mokhtar Hamitou, Laid Dehimat and M. Mourad Senoussi. 2016. Isolation of Tomato Fruits Mycoflora and Evaluation in vitro and in vivo by Trichoderma harzianum.Int.J.Curr.Microbiol.App.Sci. 5(10): 708-717. doi:
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