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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(10): 698-707
DOI: http://dx.doi.org/10.20546/ijcmas.2016.510.076


Isolation and Identification of Some Dark Septate Endophytic Fungi (DSE) from the Roots of the Ethno-Medicinal Plant Plumbago zeylanica L.
Avishek Sarkar1 and Sikha Dutta*
CAS Department of Botany, The University of Burdwan, Burdwan-713104, West Bengal, India
*Corresponding author
Abstract:

In present study an attempt has been made to isolate and identify  the dark septate endophytic fungi (DSE) and their colonization in the roots of an important ethno-medicinal plant, Plumbago zeylanica L. The plant exhibited significant colonization by DSE. Two different strains of dark septate endophytic fungi were isolated from the roots of the plant. The pure cultures were established (PUB and PUD).  DNA was isolated from the Pure culture – PUB and PUD and 28s rDNA  sequencing of  D1,   Dregion of the larger sub unit was performed. Sequence analysis exhibited sequence similarity with Perenniporia sp. CBS 130020 (Gen Bank Accession Number: JX292098.1) and Aspergillus niger strain RA402 (GenBank Accession Number: GQ169753.1) respectively based on nucleotide homology and  phylogenetic analysis.


Keywords: Dark septate  endophytic  fungi;  Perenniporia sp.; Aspergillus niger; Plumbago zeylanica L.
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How to cite this article:

Avishek Sarkar and Sikha Dutta. 2016. Isolation and Identification of Some Dark Septate Endophytic Fungi (DSE) from the Roots of the Ethno-Medicinal Plant Plumbago zeylanica Int.J.Curr.Microbiol.App.Sci. 5(10): 698-707. doi: http://dx.doi.org/10.20546/ijcmas.2016.510.076