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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(10): 688-697
DOI: http://dx.doi.org/10.20546/ijcmas.2016.510.075


Evaluation of Total Phenols, Total Flavonoids and In vitro Antioxidant Activity in the Ethanolic Leaf, Seed and Root Extract of Cajanus cajan (L.) Mill sp
R.Raveena Devi*, R. Premalatha1 and R. Kayathri2
Department of Microbiology and Biochemistry, Nadar Saraswathi College of Arts and Science,Theni - 625 531, Tamil Nadu, India
*Corresponding author
Abstract:

The purpose of the study was to assess total Flavonoids (TF), total phenols (TP) and antioxidant activity (TAA) in the ethanolic leaf, seed and root extract of Cajanus cajan. Total phenolic content, Total Flavonoids content were measured by using standard procedures and free radical scavenging activities were performed by using DPPH radical scavenging activity, Hydrogen peroxide radical scavenging and phosphomolybdenum assay. The result indicated that total phenolic content in the analysed leaf, seed and root extracts ranged as 55.03mgGAE and 57.20mgGAE, 50.95mgGAE. Similarly Flavonoids content in the analyzed leaf, seed and root extracts ranged as 36.17 mgREgram, 49.43 mgREgram, and 48.32mgREgram. The outcome of the antioxidant activity were found to be dosage dependent .The IC50values for DPPH assay for ethanolic extract of leaf, seed and root were 1.407, 0.844 and 1.184mgml respectively. The highest hydroxyl radical scavenging activity reached up to 79.07% in seed 76.45% in leaf 75.21% in root extracts respectively. The highest total antioxidant potentials observed in Cajanus cajan seed extract compared to other extracts. All the assays tested, it was found that the seed extract possessed highest antioxidant activity.  These findings indicated that potential antioxidant activity were found in Cajanus cajan leaf, seed and root extracts and needs further exploration for their effective use. The high amount of TFC, TPC, and TAA in the leaf, seed and root of Cajanus cajan, makes Cajanus cajan effective free radical scavenger and a powerful source of natural antioxidants.


Keywords: Cajanus cajan,Total Phenols, Total Flavonoids, Antioxidant potential- DPPH,  H2O2, Total antioxidant capacity.
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How to cite this article:

Raveena Devi, R., R. Premalatha and Kayathri, R. 2016. Evaluation of Total Phenols, Total Flavonoids and In vitro Antioxidant Activity in the Ethanolic Leaf, Seed and Root Extract of Cajanus cajan (L.) Mill sp.Int.J.Curr.Microbiol.App.Sci. 5(10): 688-697. doi: http://dx.doi.org/10.20546/ijcmas.2016.510.075