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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(2): 154-160
DOI: http://dx.doi.org/10.20546/ijcmas.2016.502.018


Study of Virulence Genes Cag A and Vac A in Helicobacter pylori Isolated from Mansoura University Hospital Patients by Multiplex PCR
Maysaa El Sayed Zaki1*, Ahmed Elewa1, Mahmoud Abdelwahab Ali2, Ahmed Shehta2
1Clinical Pathology Department, Mansoura Faculty of Medicine, Egypt
2Gastroenterology Surgical Center, Mansoura Faculty of Medicine, Egypt
*Corresponding author
Abstract:

Helicobacter pylori (H.pylori) is associated with various upper gastrointestinal tract disorders. Virulence genes are cofactors for the pathogenicity of H.pylori. The aims of the present study were to study the prevalence of cagA and vaca genes among H.pylori strains isolated from patients with upper gastrointestinal disorders requiring endoscopic examinations and to relate the presence of these virulence genes to the clinical presentations of those patients. The study included eighty two patients complaining of upper gastrointestinal disorders requiring endoscopic examinations Biopsies were obtained from each subject and specific culture for H.pylori were performed. Multiplex polymerase chain reaction was performed for isolated H.pylori strains to identy the presence of cagA and vaca genes. Their complaints were mainly gastric ulcer (40.2%), simple gastritis (32.9%) and duodenal ulcer (26.8%). Culture of H.pylori was positive in 60.9% of samples. Virulence gene cagA was identified in 62% and VacA in 58% of H.pylori isolates. All strains that harbor vacA had also cagA with two isolates with cagA gene alone. H.pylori was isolated in significant higher percentage (P=0007) from gastric ulcer (93.9%) then duodenal ulcer (45.5%) than simple gastritis (22.2%). Both cagA and vacA were significantly (P=0.0001) associated with gastric ulcer (51.5% & 60.6% respectively) compared to other clinical finding. From this study we can conclude that H.pylori is a common pathogen associated with upper gastrointestinal tract mainly with gastric ulcer. H.pylori strains responsible for gastric ulcer were significantly harboring the caga and vaca virulence genes. These genes may predispose to severe gastric disorders. Extended large scale studies are required to find the pathogenesis of these genes in Egyptian population.


Keywords: Virulence Genes CagA and VacA, Helicobacter pylori, Multiplex PCR
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How to cite this article:

Maysaa El Sayed Zaki, Ahmed Elewa, Mahmoud Abdelwahab Ali, Ahmed Shehta. 2016. Study of Virulence Genes Cag A and Vac A in Helicobacter pylori Isolated from Mansoura University Hospital Patients by Multiplex PCRInt.J.Curr.Microbiol.App.Sci. 5(2): 154-160. doi: http://dx.doi.org/10.20546/ijcmas.2016.502.018