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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(10): 639-647
DOI: http://dx.doi.org/10.20546/ijcmas.2016.510.071


Comparative Study between Conventional Methods over Automated Methods for Speciation of Coagulase Negative Staphylococcus in a Tertiary Care Center
S.S.M. Umamagewari1*, P. Neelu Sree1, Madhurima Mukerjee2, Kalyani1 and A.S. Shameem banu2
1Department of Microbiology, Saveetha Medical College and Hospital,Thandalam, Chennai, India
2Chettinad Medical College, Chennai, India
*Corresponding author
Abstract:

Coagulase negative staphylococcus are the commensal of humans, which are increasingly being recognized as significant nosocomial pathogens, mainly in patients under long term medical care or in immunocompromised patients. Identification and speciation of CONS is becoming medically important due to its multidrug resistant patterns and to define its clinical significance. Study was carried out from of Dec 2014 till May 2015. A total of 60 isolates from various clinical specimen was collected in a tertiary care centre. Speciation was done by conventional method which included biochemical characterization and automated method like Vitek 2 GP 21342. Antibiotic sensitivity testing was done by Disc diffusion method on Muller Hinton Agar. Out of 60 isolates Staphylococcus haemolyticus (26%) was most frequently isolated, followed by S.cohnii (23%), S.xylosus (13%), S.lugdunensis (11%), S.epidermis (10%), S.stimulans (10%), S.saprophyticus (3%), S.schleiferi (3%) and S.warnerii (3%). This finding was based on the conventional method of speciating CONS. From the total of 60 isolates Vitek correctly identified S.hamolyticus (26%), s.cohnii(23%), S.epidermidis(10%) and S.saprophyticus (3%), for the remaining species like S.xylosus, S.lugdunensis, S.simulans, S.schleiferi and S.warnerii the result was ambiguous and correct identification was done based on the supplemental test suggested by the manufacturer, by the conventional method only 3% was misidentified to be S.warneri which the automated method idnetified to be S.epidermidis. Antibiotic sensitivity testing showed 36% of methicillin resistant CONS and 63% of methicillin sensitive CONS from the same 60 isolates. Resistances to other group of drugs were also studied. Emergence of various drug resistances makes it important for speciation of CONS and to study their Antibiogram pattern for treating the infection caused by these organisms. Though speciation of CONS by automated method is expensive but it is reliable and accurate than conventional method which is more cumbersome and variable results are obtained.


Keywords: CONS,Methicillin resistant CONS, Staphylococcus hemolyticus.
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How to cite this article:

Umamagewari, S.S.M., P. Neelu Sree, Madhurima Mukerjee, M. Kalyani and Shameem banu, A.S. 2016. Comparative Study between Conventional Methods over Automated Methods for Speciation of Coagulase Negative Staphylococcus in a Tertiary Care Center.Int.J.Curr.Microbiol.App.Sci. 5(10): 639-647. doi: http://dx.doi.org/10.20546/ijcmas.2016.510.071