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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(10): 626-638
DOI: http://dx.doi.org/10.20546/ijcmas.2016.510.070


A Study on Bio film Production in Staphylococci with their Antimicrobial Susceptibility Pattern in a Tertiary Care Centre
P. Neelusree*, S.S.M. Umamageswari, Daminot Pyngrope and M. Kalyani
Department of Microbiology, Saveetha Medical College and Hospital,Thandalam, Chennai, India
*Corresponding author
Abstract:

One of the reasons for organisms being capable of defending themselves from host immune systems is their capability to form biofilms. The interior of the bacterial biofilms presents greater resistance to opsonisation by antibodies and to phagocytosis, which explains the chronic character of these infections (Parsek et al., 2003). Furthermore, the information on the capacity of a clinical isolate to produce biofilm would help a clinician to evaluate the measure of its virulence and devise an appropriate treatment plan for the patient (Christensen et al., 1985). The objective of this study is to compare the biofilm production in Staphylococcus aureus and Coagulase negative Staphylococci (CONS) using Congo red agar and Tube method and also to compare antibiogram of Biofilm producers and non-biofilm producers. Among the total sample size of 497 clinical bacterial isolates, 60 isolates were Staphylococcus, out of which 46 isolates (76%) were Staphylococcus aureus and 14 (23%) were Staphylococcus epidermidis based on morphology and biochemical properties. From 46 Staphylococcus aureus isolated, 21 (47.82%) isolates were non-biofilm producers. 25 (54.34%) isolates produced biofilm as detected by Tube Method (TM),out of which, 11 (44%) biofilm producers were detected by both the Congo Red Agar (CRA) and Tube Method (TM).14(56%) biofilm producers were detected only by tube method. Among the 14 isolates of (23%) Staphylococcus epidermidis, 4 (28%) isolates were non-biofilm producers and 10 (71%) isolates were biofilm producers as detected by Tube Method (TM). Out of which, 5 (50%)  biofilm  producers were detected by  both Congo Red Agar (CRA) and Tube Method (TM). 5(50%) biofilm producers were detected only by tube method  In our study we found that,23 (92%) biofilm producers and 13 (68.42%) non biofilm producers of Staphylococcus aureus   and 7 (70%) biofilm producers and 2(50%)  non biofilm producers of Staphylococcus epidermidis, were resistant to Penicillin and biofilm producers of Staphylococcus epidermidis, showed higher resistance to Ciprofloxacin and Cotrimoxazole.


Keywords: Biofilms,Congo red agar,Tube method, Staphylococcus aureus, Staphylococcus epidermidis.
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How to cite this article:

Neelusree, P., S.S.M. Umamageswari, Daminot Pyngrope and Kalyani, M. 2016. A Study on Bio film Production in Staphylococci with their Antimicrobial Susceptibility Pattern in a Tertiary Care Centre.Int.J.Curr.Microbiol.App.Sci. 5(10): 626-638. doi: http://dx.doi.org/10.20546/ijcmas.2016.510.070