National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Black gram is one of the important pulse crops in India. Root rot of black gram caused by Macrophomina phaseolina is widely distributed in many countries and it is a devastating pathogen right from the establishment of the crop. Screening of Psuedomonas fluorescens was done against dry root rot pathogen M. phaseolina in black gram. The antagonistic activity of P. fluorescens against M. phaseolina was tested by dual culture technique. In the present study ten isolates of P. fluorescens were isolated on King’s B medium from rizhosphere soil of different crops grown at the Agricultural College and Research Institute, Killikulam. The bacteria were identified as P. fluorescens Pfkkm1 - Pfkkm10. Among the ten isolates, seven isolates reduced the mycelial growth of M. phaseolina. Pfkkm7 recorded lowest mycelial growth of 10 mm with 88.5% reduction over control. This isolates also recorded max inhibition zone of 51mm.Pfkkm9 recorded mycelia growth of 43mm with 50.91% reduction over control and inhibition zone of 21.33mm. The other isolates like Pfkkm3, Pfkkm4 and Pfkkm10 reduced the mycelial growth but not to greater extent than Pfkkm7 and Pfkkm9. The isolates Pfkkm1, Pfkkm2, Pfkkm5, Pfkkm6, did not reduced the mycelia growth of M. phaseolina. Carbendazim reduced the dry root rot incidence and recorded lesser disease incidence of 18%. Among the two P.fluorescens isolates PFKKM7 recorded lesser disease incidence of 24%. P. fluorescens isolated Pfkkm9 also reduced the root rot incidence and recorded 40%.The inoculated control recorded the maximum disease incidence of 98%.The soil application of P. fluorescens isolates Pfkkm7 at the rate of 10g/kg of soil effectively reduced the dry root of black gram (75.5%).
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