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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(2): 149-153
DOI: http://dx.doi.org/10.20546/ijcmas.2016.502.017


Poly Acrylamide Gel Electrophoretic Studies on Ampelopteris prolifera, Ophioglossum petiolatum and Marsilea minuta
S.Dominic Rajkumar1*, M.Johnson2*, T. Shibila2, Shashank Kumar Singh1,
Shobhit Kumar Srivastava1 and Ravi Pratap Gautam1
1Department of Botany, St. Andrew’s College, Gorakhpur- 273001, Uttar Pradesh, India
2Centre for Plant Biotechnology, Department of Botany, St. Xavier’s College (Autonomous), Palayamkottai, Tamil Nadu, India
*Corresponding author
Abstract:

The present study was aimed to produce a biochemical marker for the medicinally important pteridophytes viz., Marsilea minuta Linn, Ophioglossum petiolatum Hook. and Ampelopteris prolifera (Retz.) Copel. using SDS-PAGE. To produce protein marker for the selected pteridophytes SDS-PAGE was carried out following the method described by Anbalagan. A total of 18 bands with 10 varied Rf values were demonstrated in the SDS-PAGE gel system of pteridophytes. Among the three pteridophytes studied, Marsilea minuta displayed maximum number of protein bands (7) followed by Ampelopteris prolifera (6) and Ophioglossum petiolatum (5). The proteins with Rf vlaues 0.6 and 0.97 showed their unique occurrence in Ampelopteris prolifera. Similarly the proteins with Rf values 0.73 and 0.81 demonstrated their presence only in Marsilea minuta. Ophioglossum petiolatum failed to show its unqueness in the protein gel system of pteridophytes. These banding profiles can be used as biochemical and pharmacognostical marker to distinguish the medicinally important ferns from its adulterants in the pharmaceutical industries.


Keywords: Pteridophytes; Protein profile; SDS-PAGE
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How to cite this article:

Dominic Rajkumar, S., M.Johnson, T. Shibila, Shashank Kumar Singh, Shobhit Kumar Srivastava and Ravi Pratap Gautam. 2016. Poly Acrylamide Gel Electrophoretic Studies on Ampelopteris prolifera, Ophioglossum petiolatum and Marsilea minuta.Int.J.Curr.Microbiol.App.Sci. 5(2): 149-153. doi: http://dx.doi.org/10.20546/ijcmas.2016.502.017